中文摘要：

中药活性成分的快速分离分析一直是中药研究的重点和热点问题.该文建立了在线亲和固相萃取-高效液 相色谱-二极管阵列检测-四极杆飞行时间质谱技术快速筛选中药中与α-葡萄糖苷酶有结合作用的活性成分的方 法.四通阀和六通进样阀组成接口界面,收集亲和固相萃取柱中与α-葡萄糖苷酶有结合作用的活性成分,接着进 人液相色谱系统进行分析鉴定.阳性对照品（（＋）-儿茶素）和阴性对照品（水杨酸）混合物筛选确定了方法的特异 性和准确性.接着,以玉竹为研究对象,筛选出9种主要的α-葡萄糖苷酶抑制活性化合物,包括5种苯乙基肉桂酰 胺类化合物,4种双氢高异黄酮类化合物.结果表明建立的方法简便、快速、特异性强,可用于任何复杂体系中α-葡萄糖苷酶结合活性成分的筛选.

英文摘要：

Screening and analysis of bioactive compounds from natural products is a challen-ging work due to their complexity. This paper reports the hyphenation of affinity solid-phase extraction column （ASEC） using immobilized α-glucosidase and high performance liquid chro-matography-diode array detector-quadrupole time-of- flight mass spectrometry （HPLC-DAD-Q- TOF-MS） for screening and identification of α-glucosidase binding compounds from Chinese medicines. Affinity solid-phase extraction （ASE） system was hyphenated with HPLC system via a four-port switching valve and a six-port injection valve as an interface for transferring effluents from ASE column to HPLC. Positive control （（＋）-catechin） and negative control （ salicylic acid） were performed with the approach to verify its specificity and reproducibility. Subsequently,the developed system was applied to the screening and identification of α-gluco- sidase inhibitors from Polygonatum odoratum . Five phenethyl cinnamides （α-cis- feruloylocto- pamine, N - trans- p-coumaroyloctopamine,N - trans- feruloyloctopamine,N - trans-p - coumaroy-tyramine and W rans- feruloyltyramine） and four homoisof avanones （（ 3R ）-5,7-dihydroxyl-3- （2’,4’-dihydroxylbenzyl） -chroman-4-one,（ 3R ） -5,7-dihydroxyl-6-methyl-3-（ 4＇-hydroxylbenzyl）-chroman-4-one, （ 3R ） - 5,7-dihydroxyl-6-methyl-8 -methoxyl-3-（ 4 r-hydroxylbenzyl） -chroman-4- one and （3R） - 5,7-dihydroxyl-6 , 8 -dimethyl-3- （ 4r-hydroxylbenzyl） -chroman- 4-one） with a-glucosidase inhibitory activities were identified. The results were in accordance with those of ultra-filtration screening. With the online system developed, here we present a feasible, selective and effective strategy for the rapid screening and identification of enzyme inhibitors from complex mixtures.