中文摘要：

目的探讨淫羊藿苷（icariin,ICA）对裸鼠体内食管癌移植瘤生长的抑制作用,并初步探讨其机制。方法应用MTT方法和Giemsa染色法检测和观察ICA对食管癌细胞Eca-109和TE-13的体外抑制作用。构建裸鼠的食管癌细胞移植性肿瘤模型,分为3组,每组6只。实验组每只裸鼠腹腔注射50mg/kg ICA,对照组注射相同体积的生理盐水,阳性对照组腹腔注射2mg/kg顺铂,每2天1次,共14d,每3天测量肿瘤体积,实验结束后称量肿瘤质量;TUNEL染色法观察各组裸鼠肿瘤组织的组织形态变化和凋亡情况;免疫组织化学法对肿瘤组织中Fas和FasL蛋白的表达变化进行分析。ELISA法检测外周血中FasL和IFN-γ的水平。结果 ICA体外对食管癌细胞Eca-109和TE-13的增殖无明显的抑制作用。实验组和阳性对照组裸鼠肿瘤平均体积和质量与对照组比较,差异均有统计学意义（P〈0.05）。TUNEL染色法结果显示,实验组裸鼠的肿瘤组织发生了明显的凋亡,凋亡细胞的数量较对照组明显增加（P〈0.05）。免疫组织化学实验结果表明,与对照组相比,实验组裸鼠肿瘤组织中Fas、FasL的表达明显增加（P〈0.05）。ELISA实验结果显示,实验组裸鼠外周血中FasL和IFN-γ的水平较对照组明显增高（P〈0.05）。结论 ICA体外对食管癌细胞无明显增殖抑制作用,但是通过Fas的表达、FasL和IFN-γ的分泌在体内诱导食管癌细胞凋亡,从而发挥抗食管癌的作用。

英文摘要：

Objective To investigate the inhibitory effect of icariin（ICA）on the xenograft tumors growth of esophageal carcinoma and to preliminarily investigate its mechanism.Methods The MTT assay and Giemsa staining were applied to detect and observe the in vitro inhibitory effect of ICA on esophagus cancer cell lines Eca-109 and TE-13.The xenograft tumor model of nude mouse esophagus cancer cell was constructed and divided into 3groups,6cases in each group.Each mouse in the experimental group was intraperitoneally injected by ICA 50mg/kg;while the control group was injected by the same volume of normal saline and the positive control group was injected by cis-platinum 2mg/kg,once every 2days,a total of 14 days.The tumor volume was measured once per 3d.After experiment,the tumor weight was measured;the TUNEL staining was used to observe the morphological changes and cell apoptosis of tumor tissue in each group.The changes of Fas and FasL protein expression in tumor tissues were analyzed by immunohistochemistry.The FasL and IFN-γlevels in peripheral blood were tested by the ELISA assay.Results ICA exerted no obvious inhibitory effect on the proliferation of Eca-109 and TE-13 cell in vitro.The average volume and weight of xenografts tumor had statistical difference between the experimental group and the positive control group（P〈0.05）.The TUNEL staining results showed that the tumor tissues had obvious apoptosis,the number of apoptosis cells was significantly increased compared with the control group（P〈0.05）.The immunohistochemistry experimental results showed that the expression of Fas and FasL was significantly increased（P〈0.05）.The ELISA experimental results demonstrated that the FasL and IFN-γlevels of peripheral blood in the experimental group were significantly increased（P〈0.05）.Conclusion ICA had no obvious inhibitory effect on esophageal cancer cell proliferation in vitro,but could induce in vivo apoptosis through the Fas expression and secretion of FasL and IFN-γ,thus plays the role of