中文摘要：

背景与目的 丝氨酸／苏氨酸激酶15（serlnethreoninekinase15，STK15）是一种有丝分裂激酶。它的过表达出现在许多恶性肿瘤中，并与肿瘤的发生、发展有关，但其在肺癌组织中的表达和意义尚不清楚。本研究的目的是探讨STK15在肺鳞癌、腺癌中的表达及其与各临床病理特征的关系。方法 采用免疫组织化学方法检测80例肺鳞癌、腺癌及20例癌旁肺组织标本中STK15的蛋白表达，采用Westernblot和RT-PCR方法检测40例新鲜肺鳞癌、腺癌及癌旁肺组织标本中STK15蛋白及STK15mRNA的表达。结果免疫组化结果显示STK15蛋白在肺癌中的表达率为68．75％（55／80），明显高于癌旁肺组织（0／20）（P〈0．001）；STK15表达与肺癌的分化程度有密切关系（P=0．011），而与肺癌的TNM分期、组织学类型、淋巴结转移等均无明显关系（P〉0．05）。肺癌组织中STK15蛋白（P〈0．001）和STK15mRNA（P〈0．001）的相对表达量明显高于癌旁肺组织。结论STK15蛋白及STK15mRNA在肺鳞癌、腺癌中的表达明显高于癌旁肺组织。STK15蛋白的表达与肺癌的分化程度有密切关系，高分化者明显低于低分化者。

英文摘要：

Background and objective Serine threonine kinase 15 （STK15） is a kind of mitotic kinase. The overexpression of STK15 is significantly associated with carcinogenesis in many tumors, however, its expression and significance in human lung cancer are still unclear. The aim of this study is to investigate the expression of STK15 in squamous cell carcinoma and adenocarcinoma of the lung and to analyze the correlation between STK15 expression and clinicopathological factors. Methods The pattern of STK15 protein expression was detected in 44 squamous cell carcinomas, 36 adenocarcinomas and 20 paracancerous lung tissue samples by immunohistochemistry method using anti-STK15 antibody. The relative quantity of STK15 protein expression was detected by Western blot, and STK15 mRNA expression was detected by RT-PCR in 40 fresh lung cancer samples and corresponding paracancerous lung tissues. Results Positive expression rate of STK15 protein was 68.75%（55/80） in lung cancer tissues and 0 % in paracancerous controls （P〈0. 001 ）. STK15 expression was significantly related to differentiation grade of lung cancer （P= 0.011）, but not to histological classification, TNM stages or lymphatic metastasis （P〉0.05）. The relative expression levels of STK15 protein （P〈0. 001） and STK15 mRNA （P〈0. 001） in lung cancer tissues were both significantly higher than those of corresponding paracancerous lung tissues. Conclusion The expression of STK15 protein and STK15 mRNA is significantly higher in lung cancer tissues than that in paracancerous lung tissues. The expression of STK15 correlates with differentiation of lung cancer.