中文摘要：

目的探讨8-羟基二氢小檗碱对NIT-1细胞胰岛素分泌的影响及其机制。方法采用不同浓度8-羟基二氢小檗碱干预NIT-1细胞后,以放射免疫法、液体闪烁计数法、酶法,分别检测其胰岛素水平、葡萄糖利用、葡萄糖激酶（GK）活性。结果在低浓度葡萄糖（5.5mmol/L）刺激下,与空白对照组相比,加入高浓度8-羟基二氢小檗碱（1.0μmol/L）的NIT-1细胞胰岛素分泌增加、葡萄糖利用活跃,且GK酶活性增强（P〈0.05）;在高浓度葡萄糖（16.5mmol/L）刺激下,与空白对照组相比,加入不同浓度8-羟基二氢小檗碱（0.5、1.0μmol/L）的NIT-1细胞胰岛素分泌增加、葡萄糖利用活跃,且GK酶活性增强（P〈0.01或P〈0.05）。结论 8-羟基二氢小檗碱能促进葡萄糖刺激下NIT-1细胞的胰岛素分泌,其机制可能与增加NIT-1细胞葡萄糖利用、增强GK酶活性有关。

英文摘要：

Objective To investigate the effect of 8-hydroxy dihydroberberine on insulin secretion in NIT-1 cells stimulated by glucose and the possible mechanism.Methods Radioimmunoassay,scintillation counting technique and enzymatic method were used to measure the effects of 8-hydroxy dihydroberberine on insulin secretion,glucose utilization and the activity of glucokinase（GK）.Results As compared with untreated group,insulin secretion level,glucose utilization and the activity of GK in NIT-1 cells stimulated with low concentration of glucose（5.5 mmol/L）were increased significantly in 8-hydroxy dihydroberberine-treated group（1.0 μmol/L,P0.05）.Insulin secretion level,glucose utilization and the activity of GK in NIT-1 cells stimulated with high concentration of glucose（16.5 mmol/L）were increased significantly in 8-hydroxy dihydroberberine-treated group（0.5 μmol/L or 1.0 μmol/L,P0.05 or P0.01）.Conclusion 8-hydroxy dihydroberberine can promote insulin secretion of NIT-1 cells stimulated with different concentrations of glucose,which may be associated with the improvement of glucose utilization and increase in the activity of GK.