中文摘要：

本研究旨在分析钙离子（Ca^2＋）跨膜吸收途径相关基因在昆明小鼠胃肠道中的表达模式。选取12只8周龄、平均体重（30．71±2．93）g的雌性昆明小鼠，取胃、十二指肠、空肠、回肠、盲肠和结肠组织样品，利用实时定量PCR法检测维生素D依赖性钙结合蛋白（CaBP-D9k）、瞬时性受体电位通道香草酸受体6（TRPV6）和维生素D受体（VDR）mRNA表达量。结果表明：1）CaBP-D9k、TRPV6和VDR mRNA在胃内属低水平表达，而在盲肠内表达量较高；2）随着小肠的延伸，CaBP-D9k、VDRmRNA表达量逐渐降低，而TRPV6mRNA则在回肠内高水平表达；3）CaBP-D9k（P〈0．05）、VDR（P〈0．05）、TRPV6 mRNA的表达量（P〉0．05）随着大肠肠段的延伸而不同程度地下降。结果提示，CaBP-D9k、TRPV6和VDRmRNA表达量与胃肠道Ca“跨膜吸收能力存在关联性。

英文摘要：

This study was conducted to analyze the expression patterns of calcium ion trans-membranous ab- sorption related genes in gastrointestinal tract of Kunming mice. A total of 12 female Kunming mice aged 8 weeks with an average body weight of （30.71 ±2.93 ） g were used as experimental animals. Tissues of stom- ach, duodenum, jejunum, ileum, cecum and colon were collected for the analysis of vitamin D-dependent 9 ku calcium-binding protein （ CaBP-D9k）, transient receptor potential vanilloid receptor 6 （ TRPV6）, and vita- min D receptor （VDR） mRNA expression levels by RT-qPCR. The results showed as follows ： 1 ） CaBP- Dgk, TRPV6 and VDR mRNA expressed in stomach at a low level, but in cecum at a high level; 2 ） with the extension of small intestine, expression levels of CaBP-D9k and VDR mRNA were gradually reduced, while TRPV6 mRNA expressed in ileum at a high level; 3 ） with the extension of large intestine, expression levels of CaBP-D9k （P 〈0.05）, VDR （P 〈0.05） and TRPV6 mRNA （P 〉0.05） were also gradually reduced at dif- ferent levels. These results indicate that expression levels of CaBP-D9k, VDR and TRPV6 mRNA are associat- ed with the capability of Ca2＋ trans-membranous absorption in gastrointestinal tract.