中文摘要：

目的探讨氯化钴（cobaltchloride，CoCl2）后处理对缺氧缺血性脑损伤新生大鼠空间学习记忆的作用。方法生后7日龄SD大鼠66只，分为假手术组（n=16）、缺氧缺血组（HI组，n=18）、CoCl2即刻干预组（C1组，n=14）、CoCl2术后1d干预组（c2组，n=18）。Westernblot检测术后1、2、7d脑组织HIF-1α蛋白的表达。大鼠生后7周行水迷宫观察CoCl2对大鼠空间学习记忆的影响。结果HI组和Cl组HIF—1α蛋自在术后第1、2天增多，第7天则不能检测到HIF-1α蛋白。而C2组第7天仍能检测到HIF-1α蛋白表达。水迷宫实验显示C2组较HI组3—5d时平均潜伏时间明显缩短，穿越原平台区域的时间增加，空间学习记忆能力部分恢复（P〈0．05）。结论在新生鼠缺氧缺血性脑损伤时，CoCl2促进HIF—1α蛋白的持续表达，术后1α干预可有效恢复大鼠的空间学习记忆能力。

英文摘要：

Objective To determine the effect of cobalt chloride （ CoCl2 ） post-conditioning on the spatial working memory in neonatal rats with hypoxic-ischemic induced brain damage （HIBD）. Methods Sixty-six SD rats aged 7 d were randomly divided into sham operation group （ N, n = 16）, hypoxia-ischemia group （HI, n = 18 ）, CoC12administration immediately after surgery group （C1, n = 14） and CoCl2 administra- tion 1 d after surgery group （ C2, n = 18）. The left common carotid artery of rats were ligated after anesthesia followed by hypoxia. In the sham control group, the left common carotid artery was exposed but was not ligated or exposed to hypoxia. The brain tissues were harvested from the pups in each group at 1, 2 and 7 d after ligation. HIF-1 ~x expression was detected by Western blot analysis. At age of 7 weeks, pups of each group were trained to do the Morris water maze test to evaluate their spatial learning and memory abilities. Results HIF-lot expression was up-regulated in HI, C1 and C2 groups at post operation days 1 and 2, then decreased, and even not detectable in the former 2 groups at postoperative day 7, but detectable in C2 group. Morris water maze training revealed that the rats in C2 group had significantly shortened average latency time at 3 to 5 d, and improved long-term reference memory compared with that in HI group （ P 〈 0.05 ）. Conclusion improves and maintains the expression of HIF-1α in newborn rats after hypoxic-ischemic induced brain Post-conditioning with CoCI2 at 1 d after hypoxic-ischemic working memory. damage CoCI2 damage. exerts neuroprotection and restores spatial