中文摘要：

分别以芴（3环）、荧蒽（4环）和苯并[b]荧蒽（5环）为唯一碳源（1 mg/L）,采用平板划线法对某焦化厂污染土壤中的多环芳烃降解菌进行分离.通过自制的呼吸器,研究所得多环芳烃降解菌对14C-菲（5μL/100 mL 40~60 mCi/mmol）的矿化情况;通过序批试验,以煤焦油为碳源（1μL/mL）,研究这些菌对19种多环芳烃的降解情况.多次划分后,得到4种菌,经鉴定命名为博特氏菌L1、苍白杆菌L1、微杆菌L1和赤红球菌L1.经过3周的矿化实验,微杆菌L1可以将14C-菲全部矿化成14CO2,赤红球菌L1可将大约60%的14C-菲矿化成14CO2,而博德特氏菌L1和苍白杆菌L1对14C-菲无矿化作用.经过5周的降解实验,博德特氏菌L1对大多数多环芳烃表现了良好的降解作用,苍白杆菌L1和微杆菌L1对部分多环芳烃有降解作用,而赤红球菌L1培养系统中某些多环芳烃的浓度甚至有增大,这可能与其在代谢过程中产生表面活性物质有关.所得4种菌在焦化厂污染土壤的微生物修复中具有较大的应用潜力.图3表1参27

英文摘要：

Aimed at bioremediation of the soil contaminated by a coking plant,polycyclic aromatic hydrocarbons（PAHs）-degrading bacteria were isolated from the soil and their degrading properties were studied.Streak-plating method was applied to isolate the PAHs-degrading bacteria using fluorine（3 rings）,fluoranthene（4 rings） and benzo[b]-fluoranthene（5 rings） as the sole carbon source（1 mg/L）,respectively.Self-made respiration flasks were employed to study the mineralization of 14C-labelled phenanthrene（5 μL/100 mL 40~60 mCi/mmol） by the obtained bacteria.Using coal-tar oil as the carbon source（1 μL/mL）,batch experiments were performed to investigate the degradation of 19 PAHs by the bacteria.The obtained four strains of bacteria were identified as Bordetella sp.L1,Ochrobactrum sp.L1,Microbacterium sp.L1 and Rhodococcus sp.L1,respectively.After three weeks of mineralization experiments,the 14C-labelled phenanthrene was almost converted to 14CO2 by Microbacterium sp.L1,about 60% converted by Rhodococcus sp.L1,while Bordetella sp.L1 and Ochrobactrum sp.L1 were not able to mineralize 14C-labelled phenanthrene.After five weeks of batch experiments,degradation of PAHs was most significant in the system enriched with Bordetella sp.L1,followed by Ochrobactrum sp.L1 and Microbacterium sp.L1.In the system of Rhodococcus sp.L1,the concentrations of some PAHs increased,probably due to the production of biosurfactant.It was suggested that the obtained four strains of bacteria have application potential in the bioremediation of PAHs-contaminated soil.Fig 3,Table 1,Ref 27