中文摘要：

目的探讨RNA干扰技术抑制SHBG基因表达对滋养细胞增殖的影响。方法在体外通过RNA干扰技术,将SHBG si RNA转染至原代培养的人绒毛滋养细胞中,MTT法检测滋养细胞增殖情况。结果成功将SHBG si RNA转染人绒毛滋养细胞;转染后24、48、72h,SHBG si RNA转染组的细胞生长速度显著减缓,正常对照组（未经转染的细胞）、空白对照组（只转染脂质体,无si RNA）和阴性对照组（转染非特异性si RNA）的组间比较差异均无统计学意义（P〉0.05）;转染组（SHBG si RNA-I,SHBG si RNA-II,SHBG si RNA-III）之间差异均无统计学意义（P〉0.05）,各转染组分别与各对照组的组间比较差异均有统计学意义（P〈0.05）。结论 si RNA可以有效干扰人绒毛滋养细胞SHBG基因的表达,抑制滋养细胞增殖的增殖活性。

英文摘要：

Objective：To investigate the effect of silencing SHBG gene by RNA interference on proliferation of trophoblasts. Methods：Small interferening RNA（si RNA）specific-targeting SHBG gene was transfected into cultured human first trimester trophoblasts,cell proliferation was detected by MTT method. Results：SHBG si RNA was successfully transfected into human trophoblasts;In 24,48,72 h after transfection,the cell proliferation rate of transfection groups slowed down significantly;There was no significant difference between normal control group（trophoblasts without any transfection）,blank control group（transfect liposome,without si RNA）and negative control group（transfect nonspecific si RNA）（P0.05）;There was no significant difference between transfection groups（transfect SHBG si RNA-I,SHBG si RNA-II,SHBG si RNA-III respectively）（P0.05）;There was significant difference between transfection groups and control groups（P0.05）. Conclusion：Si RNA can interfere the expression of SHBG gene in trophoblasts effectively,which inhibits the proliferation of trophoblasts.