中文摘要：

无融合生殖甜菜M14单体附加系为19条染色体甜菜，其中附加1条白花甜菜第9号染色体。为了探明无融合生殖甜菜M14的细胞及胚胎学机理，介绍了一种快速、简便观察分析无融合生殖甜菜染色体标本的制片方法及操作流程。取甜菜根尖分生区部位用0．2mmol／L8．羟基喹啉预处理，经前低渗的材料需先用甲醇：冰醋酸（3：1）固定4h，再酶解去壁、后低渗，酶解时间大约0．5～2．0h。利用滴片或涂片法通过荧光显微技术观察染色体标本，可以更加清晰地观察到染色体的自然裸露状态，附加的1条白花甜菜染色体游离于栽培甜菜染色体之外，更易于识别。此方法适用于无融合生殖甜菜，可为后续的染色体微分离及Fish荧光原位杂交等提供前提条件和技术保障。

英文摘要：

The monosomic addition line chromosome of ninth corollifiora zoss ofapomixes sugarbeet M14 has 19 chromosomes, one of them is the additional In order to study the mechanism of cytology and Embryology, the wall degaradation hypotonic method of preparing samples has been Practiced in apodictic monosomic line named sugarbeet M14 line （Beta vulgaris. , L,, VV ＋ 1C, 2n = 18 ＋ 1 ）. The root meristem of Apomixes Sugarbeet was pre-treated with 0.2 mmol/L 8-hydroxyquinoline and fixed with methanol and acetic acid （3： 1 ） for 4 h, the time of Enzymatic hydrolysis was about 0.5 -2 h. During the preparation of the chromosomes in sugarbeet M14 line, the native nudity of the alien chromoso me had also been observed. The results were as follows： The Examination of the generations M14 revealed that the genomes of its plants （VV ＋ 1C, 2n = 18 ＋ 1 ） , these indicated that the monosomic addition line M14 was a faeuhative apomicts and was easy to observe. This method is adoptable to apomixes Sugarbeet, and also can provide technical support for the study of chromosome micro dissection and Fish fluorescence in situ hybridization.