中文摘要：

将绿色荧光蛋白（GFP）基因片段插入到pBI121-Lyz多克隆区，构建了重组表达载体pBIl21-Lyz-GFP。经SmaⅠ与BamHⅠ双酶切和PCR验证重组质粒，均能得到约750bp的目的片段，通过进一步测序证明，GFP基因已成功地连接到pBI121-Lyz中，且重组质粒连接方向正确。利用冻融法将重组质粒导入根癌农杆菌（Agrobacterium tumenfaciens ） LBA4404,，用叶盘法转化和田苜蓿（Medicago sativa L. cv. Xinjiang Daye），筛选出具有卡那霉素抗性的愈伤组织，经PCR扩增和荧光显微镜检测证明，重组基因已成功地转化到和田苜蓿愈伤组织中。

英文摘要：

The dual gene vector was constructed by inserting GFP gene used as selectable marker into the pBI121-Lyz. The nucleotide sequencing showed that the plasmid pBI121-Lyz-GFP was constructed successfully. Moreover, the recombinant plasmid was introduced into Agrobacterium tumefaciens LBA4404 by freezethaw method, and then transformed into the Medicago sativa L. cv. Xinjiang Daye by leaf disc method. The kanamycin-resistant transformants were obtained by kanamycin screening. And the transgenic calluses of M. sativa cv. Xinjiang Daye were detected by PCR and the observation of GFP gene under the fluorescent microscope upon excitation of blue light. The results showed that the foreign gene was integrated into the recipient genome of M. sativa cv. Xinjiang Daye and expressed normally.