中文摘要：

目的：探讨在人骨髓间充质干细胞（h BMSCs）成骨分化过程中,不同浓度尿酸（UA）对骨形态形成蛋白-2（BMP-2）表达的影响。方法：以全骨髓贴壁培养法分离h BMSCs,将生长状态良好的第3代h BMSCs分为5组,分别为空白对照组（加入完全培养基）和成骨诱导组（加入成骨诱导液及含0 mmol/L、0.2 mmol/L、0.4 mmol/L、0.8 mmol/L尿酸的完全培养基）。连续干预诱导14d后,用倒置显微镜观察细胞形态的变化,通过观察茜素红染色情况及检测碱性磷酸酶（ALP）活性进行成骨情况的检测。RT-PCR技术检测各组细胞BMP-2 mR NA的表达情况。结果：第3代h BMSCs大多为形态单一的长梭形,呈旋涡状生长;干预诱导后的细胞逐渐变成不规则的立方形,局部形成团块状结节,以含尿酸浓度为0.8 mmol/L的成骨诱导培养基最为显著。连续干预14d后,空白对照组茜素红染色为阴性,而各成骨诱导组细胞茜素红染色结果为阳性,提示干预诱导后的细胞为成骨细胞。碱性磷酸酶活性随尿酸浓度的增加和干预时间的延长而增强（P〈0.05）。RT-PCR检测结果显示,空白对照组无BMP-2 mR NA的表达。成骨诱导组随培养基中尿酸浓度的增加,BMP-2 mR NA表达逐渐增强,呈浓度依赖性（P〈0.05）。结论：尿酸上调h BMSCs向成骨细胞分化过程中BMP-2 mR NA的表达。

英文摘要：

Objective： To observe effect of uric acid （UA） on expression of bone morphogeneUc protein-2 （BMP-2） in differentiation process from human bone marrow mesenchymal stem cells （hBMSCs） to osteoblasts in vitro. Methods： hBMSCs were isolated and cultured using the whole bone marrow adherence method. Passage 3 hBMSCs were divided into 5 groups： blank control group （adding complete medium） and osteogenic induction group （adding osteoblast inducing media and 0 mmol/L, 0.2 mmol/L, 0.4 mmol/L, 0.8 mmol/L UA in complete medium）. After 14 days of induction, cell morphology was observed under an inverted microscope. Osteogenic ability was identified by alkaline phosphatase （ALP） activity assay and alizarin red stain. BMP-2 mRNA expression was detected by reverse transcription PCR （RT-PCR）. Results： Passage 3 cells were single long-spindle in shape and gradually formed a whirlpool-shaped arrangement. After induction, the majority cells were from long-spindle to irregular cube in shape, and gradually became paving stone or formed nodules. Among all the groups, the cells in 0.8 mmol/L UA induction medium formed the most nodules. After 14 days of induction, calcium nodules in the blank control group were negtive, however, calcium nodules were dyed orange in osteogenic induction group. The result showed hBMSCs were induced into osteoblasts successfully. ALP activity enhanced gradually with the increase of intervention time and UA concentration （P〈0.05）. RT-PCR results showed that BMP-2 mRNA was hardly detected in control group. In induced group, the mRNA expression of BMP-2 was evaluated with the increase of UA concentrations （P〈0.05）. Conclusions： UA can upregulate expression ofBMP-2 mRNA during osteogenic differentiation ofhBMSCs.