中文摘要：

笔者使用6种DNA提取方法提取7种含糖量较高的石斛属植物的DNA,比较不同方法提取DNA的效果,结果表明：改良PEX法和改良CTAB法对含有丰富多糖和多酚类的石斛属植物的DNA提取效果较好.改良PEX法与其他5种方法对比,可以更好地去除多糖类和多酚物质的干扰,能够满足后续的分子生物学实验的要求.改良CTAB法和改良SDS法与CTAB法和SDS法相比,可获得较高的DNA产率,并且可以较好地去除多糖类杂质的干扰.

英文摘要：

Extracting high quality DNA is very important for molecular experiments. Dendrobium species under the family Orchidaceae contain more sucrose and polyphenol substances and, it is hence difficult to extract high quality DNA from Dendrobium species. Six methods were used to extract DNA from 7 Dendrobium species, and compared to develop an optimal extracting method for DNA. Both PEX-improved method and CTAB-improved method had positive effect on DNA extraction from Dendrobium spp. which contained more polysaccharides and polyphenols. Compared with other 5 methods, the PEX-improved method removed higher content of polysaccha- rides and phenolics from Dendrobium spp and met the need of molecular biology experiments. The CTAB-im- proved method and the SDS-improved method removed more content of polysaccharides, and produced higher yield DNA samples as compared with the CTAB method and the SDS method.