中文摘要：

目的探究丁苯酞(NBP)对慢性酒精中毒大鼠学习记忆能力、海马内硫化氢(H2S)和纤维状肌动蛋白(F-actin)含量的影响。方法将50只SD雄性大鼠随机分为5组,除对照组外,将含酒的水作为大鼠唯一水来源,并逐渐增加酒精含量,采用自由饮用方法建立慢性酒精中毒模型。42 d后按慢性酒精中毒大鼠戒断评分表于末次饮酒后6 h检测大鼠模型,NBP低、中、高剂量组大鼠灌胃不同剂量NBP(大约14 d),饮酒结束后利用Y型电迷宫测试酒精中毒大鼠的学习记忆能力,随后检测海马组织H2S和F-actin的含量。结果与对照组大鼠学习记忆成绩(40.38±5.97)、H2S含量(23.25±3.61)和F-actin在阳性细胞中的表达相比,模型组大鼠学习记忆成绩(83.25±5.47)和H2S含量(43.86±6.95)均升高,F-actin的阳性表达降低(P<0.01)。与模型组大鼠相比,NBP中、高剂量组大鼠学习记忆成绩(64.75±4.71)、(70.00±4.72)和H2S含量(32.21±5.32)、(36.78±7.29)nmol/g均降低,F-actin的阳性表达升高(P<0.01)。结论 NBP能够减轻慢性酒精中毒大鼠对酒精的依赖,缓解酒精对大鼠学习记忆能力的影响,可能与NBP影响H2S和F-actin的含量有关。

英文摘要：

Objective To investigate the effect of butylphthalide ( NBP) on the learning and memory abilities of chronic alcoholism rats,H2ScontentandtheexpressionofF-actininthehippocampusofchronicalcoholismrats.Methods 50maleSDratswererandomlydi-vided into five groups .in addition to control group ,the rats were reared by the water containing wine .The alcohol content was gradually in-creased to establish the free drinking modeling .After 42 d, 6 h after drinking rat models were detected by chronic alcoholism withdrawal score sheet.The low,medium and high dose NBP group rats were fed different doses of NBP ,the learning and memory were tested bya Y-shaped maze detection the H 2 S content of the hippocampus and the expression of F-actin were measured .Results Compared to learning and memory scores(40.38±5.97),H2S content(23.25±3.61)and the expression of F-actin in control group rats,learning and memory scores (83.25±5.47) and H2S content (43.86±6.95) in rats of model group were increased and F-actin positive expression was reduced (P<0. 01).Compared with those of model group , learning and memory scores (64.75±4.71) (70.00±4.72) and H2S content (32.21±5.32) (36.78±7.29) in medium, high dose NBP group were reduced and F-actin positive expression was increased (P<0.05).Conclusions NBP could reduce of chronic alcoholism rat alcohol dependence , and mitigate the impact of alcohol on learning and memory abilities of rats with NBP might affect H 2 S and F-actin contents relevant .