中文摘要：

目的：为了研究PGC-1α对小鼠运动耐力的调控作用,并探讨PGC-1α与其上游调控信号的关系,进一步阐明PGC-1α在不同骨骼肌运动过程中的分子机制。方法：将18只C57BL/6雄性小鼠按体重和耐力分为安静组、运动开始后1 h组和运动力竭组,进行预适应后以15 m/min的速度对小鼠进行运动耐力测试,直至小鼠运动力竭。检测腓肠肌、比目鱼肌和跖肌中的PGC-1α及p-AMPKα表达的变化,分析二者之间的相关系数。结果：与安静组的PGC-1α及p-AMPKα表达相比,腓肠肌运动开始后1 h组的表达均显著升高,运动力竭组表达迅速降低,且腓肠肌内PGC-1α及p-AMPKα表达呈显著相关,相关系数为0.910。比目鱼肌表达呈逐渐上升趋势,并维持在较高水平,比目鱼肌内表达呈显著相关,相关系数为0.929。跖肌表达则呈显著负相关,相关系数为-0.879。结论：p-AMPKα作为骨骼肌内PGC-1α的上游调节信号,其升高有助于肌纤维的动员及运动耐力的提升,可考虑外源性用药调控腓肠肌和比目鱼肌中的AMPK激活PGC-1α以提高相关机能。

英文摘要：

Objective： To research the regulation of PGC-1α to endurance exercise, discuss the relationship be-tween PGC-1 α and upstream signals, and further clarify the molecular mechanism of PGC-1 α in different skeletal muscle during the exercises. Methods： Eighteen C57BL/6 mice were divided into Pre, 1 h after start, and Post by weight and endurance. The endurance exercise was 15 m/min after adaptation until the exhaustion. Test the PGC-1 α and p-AMPKα of gastrocnemius, soleus, and plantaris, analyze the correlation coefficient between them. Re suits： Compared to the PGC-1 α and p-AMPKα of Pre, 1 AS of gastrocnemius express increased significantly. The expression of Post decreased rapidly, which had significantly correlation, the coefficient was 0. 910. Soleus ex-pression was gradually increasing, and maintained at high levels. It had significant correlation inside, the coeffi-cient was 0. 929. Plantaris expression was a significant negative correlation, the coefficient was - 0. 879. Conclu-sion ： As upstream signals in skeletal muscle, the increase of p-AMPKα could promote the mobilization and endur-ance, and the exogenous use of drug could regulate the PGC-1 α and p-AMPKα of gastrocnemius and soleus to im-prove the relevant function.