中文摘要：

目的研究病毒滴度和插入子长度对于慢病毒载体介导的转基因小鼠整合率的影响。方法制备了18种不同长度插入子的慢病毒载体，通过受精卵卵周隙注射的方法获得转基因小鼠，然后通过PCR技术检测其转基因小鼠的整合率，并对所生成的数据进行统计学分析。结果插入子长度在较短（〈2kb）的情况下，病毒滴度达到2×10^8就可获得较高的整合率，在插入子长度稍长（4-5kb）的情况下，需要1×10^9滴度才能获得较高的整合率，而在插入子长度大于6kb的情况下，未能用该方法获得转基因小鼠。结论滴度的增加能有效提高慢病毒载体制备转基因小鼠的整合率，而当滴度适中（-2×10^8）时，整合率随插入子长度的增加而下降。

英文摘要：

Objective To study the effeet of viral particle titer and the transgene length on the integration rate of transgenic mice mediated by lentiviral vector particles. Methods Eighteen kinds of lentiviral vectors particles, which carded various lengths of inserts, were used for subzonal injection of mouse fertilized eggs, followed with transplanting those eggs into the oviduct of pseudopregnanting mice. Neonates were then identified by PCR analysis. Results For short inserts （〈2 kb）, only 2 x l0s of virus titer was needed to achieve a high integration rate. While the length of inserts were increase （4-Skb）, 1 × 10^9 viral particles were required for having a reasonable integration rate. However, transgenic mice was not able to be achieved when the inserts were longer than 6kb, in our hands. Conclusion Integration rate of transgenic mice was largely affected by both the viral titer and the length of inserts. It increases with the particle titer, while decreases with the length of inserts