中文摘要：

目的 分析人巨细胞病毒（HCMV）merlin株全基因组存在ORF重叠的基因,揭示HCMV重叠基因的结构与功能特征.方法 应用生物信息学方法 搜索HCMV Merlin全基因组中的ORF重叠基因,分析HCMV merlin株重叠基因的编码序列CDS和ORF起止位点,计算CDS和ORF的长度,编码蛋白的相对分子质量,重叠长度,蛋白编码方向,确定重叠序列,重叠类型,分析重叠基因的表达时相和编码蛋白的功能.结果 HCMV merlin株存在39个重叠ORF的基因,占全基因总数的23%,39个ORF重叠基因之中有13个IE基因,9个E基因,17个L基因,可分为16个重叠群,2个基因重叠的有11个重叠群,3个基因相互重叠的有3个重叠群,4个基因相互重叠的有2个重叠群,重叠基因功能广泛.结论 HCMV Merlin株中存在大量的复杂的重叠基因,为进一步研究重叠基因的转录和翻译调控机制奠定了基础.

英文摘要：

Objective To determine the genes in which exist overlapping ORF in Merlin strains of human cytomegalovirus, and to reveal their structure and functional characteristics. Methods We search for overlapping genes of ORF in HCMV Merlin strains＇ whole genome by Bioinformatics methods, analyzing coding sequence CDS and starting and ending sites of ORF, calculating the length of CDS and ORF, analyzing the molecular weight of encoding protein, overlapping length and coding direction of protein, identifying overlapping sequences and overlapping types, analyzing the expression phase of overlapping genes and the function of proteins. Results There were 39 overlapping ORF genes in HCMV Merlin strains, accounting for 23% of total genes. Among these 39 genes, there are 13 IE genes, 9 E genes and 17 L genes, which can be divided into 16 contigs. There are 11 contigs when two genes overlap, with 3 contigs in three genes overlapping, and 2 contigs in four genes overlapping. The functions of overlapping genes are widely. Conclusion We found that there are a lot of complex overlapping genes in HCMV Merlin strains, which are basis for further study of the transcription and translation mechanism of overlapping genes.