中文摘要：

磷酸泛酰巯基乙胺基转移酶（PPTase）催化脂肪酸合酶（FAS）、聚酮合酶（PKS）和非核糖体肽合成酶（NRPS）中载体蛋白从脱辅基形态转化为全辅基形态，对脂肪酸、PKS产物和NRPS产物的生物合成起着不可或缺的作用。本文介绍并总结了链霉菌PPTase对载体蛋白底物选择性的最新研究进展：Ⅲ型PPTase特异性催化同一个多肽链中ACP的辅基化；II型PPTase倾向于催化Ⅰ型PKS中ACP和NRPS中PCP的辅基化；Ⅰ型PPTase倾向于催化Ⅱ型PKS中ACP和Ⅱ型FAS中ACP的辅基化；编码基因位于基因簇内的Ⅰ型／Ⅱ型PPTase倾向于催化编码基因位于同基因簇内的PKS／NRPS中ACP／PCP的辅基化；这些研究结果为阐明并改造链霉菌辅基化网络以提高特定次级代谢产物的产量提供了参考和借鉴。

英文摘要：

Phosphopantetheinyl transferases （PPTases） play essential role in the biosynthesis of fatty acids, polyketides, and nonribosomal peptides. PPTases phosphopantetheinylate carrier proteins in polyketide synthases （PKSs）, fatty acid synthases （FASs） and nonrihosomal peptidesynthetases （NRPSs）. In this review, we discuss current studies on the substrate specificities of PPTases to carrier proteins in Streptomyces. The substrates of type Ⅲ PPTases are acyl carrier proteins （ACPs） which locate within the same PKSs. The favorite substrates of type Ⅰ PPTases are ACPs in type Ⅱ FASs and type Ⅱ PKSs. The favorite substrates of type ⅡPPTases are ACPs in type Ⅰ PKSs and peptidyl carrier proteins （PCPs） in NRPSs. The favorite substrates of a type Ⅰ / Ⅱ PPTase, whose encoding gene locates in a gene cluster, are ACPs/PCPs whose encoding genes locate in the same gene cluster.