我们使用了倡导者陷井技术识别米饭植物,说出 107 # ,在哪个 beta-glucuronidase (GUS ) 记者基因在内乳明确地被表示。T-DNA 的 Asingle 拷贝被插入到植物染色体,并且候选人基因 OsRRM 被插入识别。OsRRM 倡导者在米饭内乳明确地指导了 GUS 表示,类似于在 107 观察的 GUS 表示模式 # 。OsRRM 是在米饭的单个拷贝的基因并且编码与二个 RNA 识别主题和一根 Spen 帕拉木头和 ortholog C 终端领域包含 1005 氨基酸的残余的原子蛋白质。西方的污点分析证实那 theOsRRM 蛋白质明确地在米饭内乳被表示。在转基因的植物的 OsRRM 的宫外的表示导致了畸形,例如短身高,延迟的生长和低结果实率。我们的数据与 Spen 基因的报导函数一起,在在米饭内乳的房间开发的规定的 implicatedOsRRM。
We used the promoter trap technique to identify a rice plant, named 107^#, in which the β-glucuronidase (GUS) reporter gene was expressed specifically in the endosperm. A single copy of the T-DNA was inserted into the plant genome, and a candidate gene OsRRM was identified by the insertion. The OsRRM promoter directed GUS expression specifically in rice endosperm, analogous to the GUS expression pattern observed in 107^#. OsRRMis a single-copy gene in rice and encodes a nuclear protein containing 1 005 amino-acid residues with two RNA recognition motifs and one Spen paralog and ortholog C-terminal domain. Westem blot analysis confirmed that the OsRRM protein was specifically expressed in rice endosperm. Ectopic expression of OsRRM in transgenic plants led to abnormalities, such as short stature, retarded growth and low fructification rates. Our data, in conjunction with the reported function of Spen genes, implicated OsRRM in the regulation of cell development in rice endosperm.