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中文摘要：
利用DGGE、定量PCR以及16S rRNA基因克隆文库技术对洋山深水港海域表层海水中的古菌多样性组成进行了调查和分析.通过Quantity One软件分析不同样品的DGGE图谱,发现洋山深水港3个不同海域（小洋山港口区、大洋山港口区、中间航道）的古菌群落多样性组成不存在明显的区别;利用定量PCR的方法对3个不同样品中的古菌16S rRNA基因拷贝进行计数,发现3个不同海域的古菌16S rRNA基因拷贝数的数量关系为：大洋山海域（（1.69±0.19）×106 copies/mL）＜中间航道（（2.17±0.20）×106 copies/mL）＜小洋山港口区（（2.42±0.22）× 106 copies/mL）,这说明洋山深水港3个不同海域的古菌群落组成是一致的,只是在数量上略有差异,为对该海域古菌的更深入调查研究提供了便利和数据基础.洋山深水港海域表层海水的古菌16S rRNA基因文库分析表明,古菌由三大门类组成：广古菌门（Euryarchaeota）、泉古菌门（Crenarchaeota）和奇古菌门（Thaumarchaeota）.广古菌门所占比例约为26.8％,泉古菌门约为33.0％,奇古菌门约为40.2％.广古菌门含有2个类群,分别是Mathanobacteriales（10.3％）和Marine Group Ⅱ（16.5％）;泉古菌门含有1个类群,为Unidentified Crenarchaeotic Group（33.0％）;奇古菌门含有1个类群,为Marine Group Ⅰ （40.2％）.结果显示,Mathanobacteriales类群和Marine Group Ⅱ类群的比例失调,这可能预示着洋山深水港海域存在油污污染及外来微生物物种入侵的风险.
英文摘要：
The archabacterial diversity in surface seawater of Yangshan deep-water port （YDWP） were investigated and analyzed using DGGE,quantitative PCR,16S rRNA gene cloning library technique.DGGE atlas of different samples was analyzed through Quantity One software; and it was found that there were no noticeable differences among the archabacterial community diversity constitution in three different sea areas of YDWP （Xiao Yangshan Port area,Da Yangshan Port area,and Middle passage） ; counting of 16S rRNA gene copies of three samples using quantitative PCR had found that the quantity relation of three different sea areas was as follow：Da Yangshan area （（1.69 ±0.19） × 106 copies/mL） ＜ Middle passage （（2.17 ±0.20） × 106 copies/mL） ＜ Xiao Yangshan area （（2.42 ±0.22） × 106 copies/mL）,this explained that the constitution of archabacteria in three different sea areas of YDWP was identical,only had slight differences in the quantity,these had provided some convenient and data bases for deep investigation and study of archabacteria in the sea areas in the future.16S rRNA gene library analyses showed that archabacteria of surface sea water of YDWP consisted of three major phylums：Euryarchaeota （26.8％）,Crenarchaeota （33.0％）,and Thaumarchaeota （40.2％）,respectively; Euryarchaeota contained two groups：Mathanobacteriales （10.3％） and Marine Group Ⅱ （16.5％） ; Crenarchaeota one group：Unidentified Crenarchaeotic Group （33.0％） ; and Thaumarchaeota one group：Marine Group Ⅰ （40.2％）.Archabacterial community structure of three different areas from YDWP was the same except for a slight difference of the number of archabacterial 16S rRNA genes.The results showed that the proportion disorder of Mathanobacteriales and Marine Group Ⅱ may indicate a potential risk about the oil pollution and foreign microbial species invasion in YDWP.
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