利用Biolog研究温带森林次生演替过程中土壤微生物多样性变化,结果表明:就总体AWCD值来看,阔叶林土体土微生物功能多样性较高于原始红松林,而在根际土上反映更加明显,阔叶林红松幼苗根际土AWCD值几乎高出原始红松林25%。而就整体丰富度及多样性指数上来看,原始红松林根际无论在多样性还是丰富度上均占优,但其土体土却均处于最低位置,这表明在原始林中,微生物更加集中于根系附近。通过单个碳源方差分析发现,在原始林中,无论是土体土还是根际土,在对氨基酸类碳源的利用上均有较大优势。这表明在温带森林次生演替过程中土壤微生物多样性确有变化,且主要集中在根际部分。
The changes of soil microbial diversity in the process of secondary succession of temperate forest were studied with Biolog.The results showed that,as to the value of the overall AWCD,microbial functional diversity of broad-leaved forest soil was slightly higher than that of the original Korean pine forest.And it was more evident to the rhizosphere soil,as AWCD values of rhizosphere soil in Broad-leaved Korean pine seedlings were almost 25% higher than in original Korean pine forest.In regard to the overall richness and diversity index,the rhizosphere of original Korean pine forest was dominant both on diversity and abundance,but its bulk soil was at the lowest level,which indicated that,in primary forests,micro-organisms were more concentrated in the vicinity of roots.Through variance analysis of single carbon source,it found that,in the original forest,whether bulk soil or rhizosphere soil,utilization of Amino acid carbon both were more advantageous.This indicated that in the secondary succession of temperate forest,the soil microbial functional diversity did have been changed and focused in the rhizosphere.