中文摘要：

目的：建立大鼠实验性牙髓炎模型，了解Cryopyrin在大鼠牙髓组织的表达情况。方法：30只Sprague—Dawley大鼠随机分为0，1，3，5，7d5组。除0d组外，其余四组大鼠双侧下颌第一磨牙开髓后暴露于VI腔环境中，分别于开髓后1，3，5和7d处死大鼠，分离双侧下颌骨。常规组织学处理后HE染色观察牙髓炎症状况，免疫组化检测Cryopyrin在牙髓炎中的表达及定位。结果：大鼠正常牙髓组织成牙本质细胞层Cryopyrin阳性表达，牙髓内部血管内皮细胞偶见表达，牙髓成纤维细胞未见表达。炎性牙髓组织血管内皮细胞表达增加，牙髓成纤维细胞及多种炎症细胞均有阳性表达，且表达的数量和牙髓的炎症浸润强度显著正相关（r=0．655，P〈0．01）。结论：Cryopyrin在牙髓组织中表达并在牙髓炎的发生发展中起着重要作用。

英文摘要：

To investigate the expression and localization of Cryopyrin in the development of experimental pulpitis in rat. Methods： Thirty Sprague-Dawley rats were randomly assigned into five groups according to the excution day. The pulps of bilateral mandibular first molar were ex- posed in the oral environment except the rats of day 0 group. The animals in the other groups were sacrificed at on the 1st, 3rd, 5th and 7th days respectively after pulpal exposure. The bilat- eral mandibles samples were extracted for histological processing, then they were stained by he- matoxylin-eosin （HE） to examine inflammation infiltration in the pulp, as well as immunohisto- chemcally examined for the Cryopyrin. Results： Cryopyrin was detected in the cytoplasm of odon- toblasts and vascular endothelial cells, but not in the fibroblasts of the normal pulp. In the in- flammatory pulp tissue, fibroblasts, monocytes, neutrophils, macrophages and vascular endothe- lial cells all expressed Cryopyrin. The number of Cryopyrin-positive cells was significantly corre- lated with the intensity of inflammatory infiltration （r=0. 655, P〈0.01）. Conclusion： Cryopyrin is expressed in the normal and inflammatory pulp tissues, and plays an important role in the de- velopment of pulpitis.