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中文摘要：
为研究叶酸和VB_（12）协同作用对中华绒螯蟹（Eriocheir sinensis）幼蟹生长、非特异性免疫和抗病力的影响,选取初始体重为（2.57±0.03）g的幼蟹600只,随机分成4组,每组5个重复,每个重复30只幼蟹,分别投喂对照组（不添加叶酸和VB_（12））,单一VB_（12）组（0.2 mg/kg）,单一叶酸组（2.3 mg/kg）和联合处理组（0.2 mg/kg VB_（12）＋2.3 mg/kg叶酸）的饲料8周。在养殖实验结束后,先统计成活率和称重,然后从每个处理组随机选取30只幼蟹,用2×10~8 CFU/m L的嗜水气单胞菌注射攻毒2周。实验结果表明：幼蟹的增重率、特定生长率、饲料效率和存活率在联合处理组最高,显著高于对照组（P〈0.05）,但与单一叶酸或VB_（12）组相比不存在显著差异（P〉0.05）。联合处理组的血清酚氧化酶活性显著高于对照组（P〈0.05）,但与单一叶酸或VB_（12）组也无显著性差异（P〉0.05）。同时,联合处理组的血清酸性磷酸酶、碱性磷酸酶、溶菌酶活性和血细胞总数等指标最高,其次是单一叶酸组和VB_（12）组,而对照组最低。投喂联合处理组饲料幼蟹的肝胰腺超氧化物歧化酶活性最高,而丙二醛含量和累积死亡率最低。以上结果表明,叶酸和VB_（12）对幼蟹的生长、生理代谢和免疫性能均可能有互补和协同作用,养殖生产中建议饲料中叶酸和VB_（12）添加量分别为2.3 mg/kg和0.2 mg/kg。
英文摘要：
In this study, we aimed to determine the combined effects of dietary folic acid and vitamin B_（12） on the growth, non-specific immunity, and the disease resistance of juvenile Chinese mitten crab Ericheir sinensis. The crabs [initial weight,（2.57 ± 0.02） g] were fed with 4 experimental diets containing 2 concentrations of folic acid（0 and 2.3 mg folic acid/kg diet） and vitamin B_（12）（0 and 0.2 mg vitamin B_（12）/kg diet）. Each diet group had 5 replicates and was fed for 8 weeks. This was followed by an Aeromonas hydrophila challenge for 2 weeks. E. sinensis fed with 2.3 mg folic acid/kg and 0.2 mg vitamin B_（12）/kg showed significantly higher weight gain, specific growth rate, feed efficiency and survival rate than those fed without folic acid and vitamin B_（12） supplementation. The activity of phenoloxidase was the maximum in juveniles fed with 2.3 mg folic acid/kg and 0.2 mg vitamin B_（12）/kg. Juveniles fed with both folic acid and vitamin B_（12） supplementation had the highest levels of total haemocyte count, lysozyme, and activities of acid phosphatase and alkaline phosphatase, followed by crabs fed with either folic acid or vitamin B_（12） only, and the group without folic acid or vitamin B_（12） supplementation was the lowest. Crabs fed with 2.3 mg folic acid/kg and 0.2 mg vitamin B_（12）/kg exhibited the highest activity of superoxide dismutase but the lowest level of malondialdehyde and the cumulative mortality. These results indicated that folic acid and vitamin B_（12） had an interactive effect on the growth, non-specific immunity and the disease resistance of juvenile E. sinensis. The optimal concentrations of dietary folic acid and vitamin B_（12） should be 2.3 mg/kg diet and 0.2 mg/kg diet respectively.
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