中文摘要：

目的建立瞬时受体电位家族香草醛1型受体（Trpv1）转基因小鼠模型。方法通过显微注射法把pcDNA3.1-rTrpv1导入C57BL/6J×CBAF1小鼠的受精卵雄原核中并移植到假孕母鼠输卵管内,用PCR方法鉴定子代基因型,用蛋白免疫印迹法检测TRPV1在组织中的表达水平。结果将400枚注射卵移植到16只假孕母鼠的输卵管中,共出生小鼠120只,获得5只转基因阳性小鼠。其中2只阳性小鼠可稳定传代,经过与C57小鼠回交数次后各自建系。经蛋白免疫印迹法证实,与同窝野生型小鼠相比,转基因小鼠的脑组织和肌肉组织中TRPV1蛋白的表达显著升高。结论成功建立了Trpv1转基因小鼠模型,为深入研究TRPV1通道的功能提供了更好的条件。

英文摘要：

Objective To establish a transgenic mouse model with the overexpression of transient receptor potential family vanilloid subtype 1 （ Trpvl ）. Methods peDNA3, 1-r Trpv1 was injected into the pronueleus of fertilized ooeytes from C57BL/6J×CBA F1 mice. The injected pronucleus were transplanted into the oviduct of pseudopregnant mice. Tail DNA PCR screening were performed to identify the positive founder mice. The expression of TRPV1 protein in the tissues of the transgenie mice was detected by Western blotting. Results 400 zygote cells were transplanted into the oviducts of 16 recipients. 120 viable offspring were born from 12 of the recipients. Tail DNA PCR screening showed 5 of the offspring were positive transgenic mice. As detected by Western blotting,TRPV1 expression in the brain and skeletal muscle of the transgenic mice was significantly higher than the wild-type littemates.Conclusion The Trpvl transgenic mouse roodel has been established successfully. The model will contribute to the further research of the physiological function of TRPV1 channels.