中文摘要：

Planktonic 细菌充满 Chukchi 边界地方区域。然而，很少在海洋对他们的差异和各种各样的细菌的角色被知道。海水样品从海冰显然正在融化的二车站 K2S 和 K4S 被收集。有在 situ 杂交(鱼) 的荧光的水样品的分析证明降级 DMSP 细菌在车站 K2S 说明了 13% 全部的细菌。没有氧气的 anoxygenic phototrophic (AAP ) 细菌在两件样品被检测。细菌的社区被二个 16S rRNA 基因克隆图书馆描绘。序列掉进领域细菌的四个主要的系，包括 Proteobacteria (Alpha，贝它和 Gamma 子类) ， Bacteroidetes， Actinobacteria 和 Firmicutes。没有重要差别在二个克隆图书馆之间被发现。Alphaproteobacteria 的 SAR11 和 Rhodobacteraceae clades 和 Gammaproteobacteria 的 Pseudoalteromonas 在克隆图书馆组成了三主导的部分。191 不是自养的细菌的紧张的一个总数被孤立， 76% 出现了细胞外的解朊的活动。种系发生的分析表明 isolates 掉进 Gammaproteobacteria， Bacteroidetes， Actinobacteria 和 Firmicutes。在两个的最普通的类细菌孤立并且生产朊酶的细菌是 Pseudoalteromonas。UniFracdata 在在 Chukchi 边界地方和北 Bering 海之间的细菌的社区显示出暗示差别。

英文摘要：

Planktonic bacteria are abundant in the Chukchi Borderland region. However, little is known about their di- versity and the roles of various bacteria in the ocean. Seawater samples were collected from two stations K2S and K4S where sea ice was melting obviously. The analysis of water samples with fluorescence in situ hybridization （FISH） showed that DMSP-degrading bacteria accounted for 13% of the total bacteria at the station K2S. No aerobic anoxygenic phototrophic （AAP） bacteria were detected in both samples. The bacterial communities were characterized by two 16S rRNA gene clone libraries. Sequences fell into four major lineages of the domain Bacteria, including Proteobacteria （Alpha, Beta and Gamma subclasses）, Bac- teroidetes, Actinobacteria and Firmicutes. No significant difference was found between the two clone li- braries. SAR11 and Rhodobacteraceae clades of Alphaproteobacteria and Pseudoalteromonas of Gammapro- teobacteria constituted three dominant fractions in the clone libraries. A total of 191 heterotrophic bacterial strains were isolated and 76% showed extracellular proteolytic activity. Phylogenetic analysis reveals that the isolates fell into Gammaproteobacteria, Bacteroidetes, Actinobacteria and Firmicutes. The most common genus in both the bacterial isolates and protease-producing bacteria was Pseudoalteromonas. UniFrac data showed suggestive differences in bacterial communities between the Chukchi Borderland and the northern Bering Sea.