中文摘要：

目的建立一种蛋白质-DNA相互作用的新型压电生物传感器检测平台，并应用于NF—κBp65蛋白的实时检测。方法AT切型基频10MHz的镀金膜正方形石英晶体晶片作为传感元件，制作2×5型传感器检测微阵列，巯基修饰的NF—κB顺式作用元件序列探针通过分子自组装技术固定在阵列各检测池的金膜电极上，实时检测NF—κB的p65结合亚基浓度。结果构建的蛋白质-DNA传感器在气相条件下，检测频率在30min内的变化为±1Hz，液相条件下检测频率变化为±2Hz；随着蛋白浓度的增加，p65蛋白与DNA探针结合反应所引起的传感器频率变化逐渐增加，当蛋白浓度为40μg／出时，频率变化响应值最大。结论成功构建了蛋白质-DNA相互作用新型压电生物传感器检测平台，并实现了对NF—κBp65的实时检测。

英文摘要：

[ Objective ] To establish a novel piezoelectric biosensor detection platform based on protein-DNA interaction and evaluate the concentration of NF-κB p65 for real-time detection by the system. [ Methods ] AT-cut 10 MHz foursquare quartz crystals with gold electrodes were used to construct the 2 ~ 5 model of piezoelectric biosensor detection array. The cis-acting element probe of NF-κB modified by thiol group was immobilized on the gold membrane surface of the quartz crystal sensor by thiol-self-assembly technique and the concentrations of NF-κB p65 subunit were real-time detected by this system. [ Results ] In 30 minutes, the changes of frequency were less than ± 1 Hz in gas phase and ＋ 2 Hz in liquid phase, respectively. With increasing concentration of protein, frequency changes of the piezoelectric biosensor caused by binding reaction of p65 protein and DNA probes were increasing gradually. When the protein concentration of NF-κB p65 subunit was 40 ng/μl, the frequency change for the response to p65 and DNA probe binding was the greatest value. [ Conclusion] The novel piezoelectric biosensor detection platform based on protein-DNA interaction have been constructed successfully and provided the real-time detection of the NF-κB p65 protein.