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中文摘要：
目的探讨Notch-1在人胶质瘤细胞系U251细胞中的邻分泌机制。方法用免疫荧光组织化学方法检测单个孤一和聚集相邻的两个U251细胞的Notch-1及配体delta-like-1的表达水平。封闭U251细胞的Notch-1受体，采用SYBR荧光实时定量PCR法检测Notch-1配体delta-like-1mRNA在不同封闭时间组的表达水平。结果聚集相邻的U251细胞Notch-1及delta-like-1的平均荧光强度高于单个孤一的细胞。封闭后delta-like-1mRNA的表达水平明显呈时间依赖性降低。结论在U251胶质瘤细胞中，Notch-1可能通过邻分泌机制促进相邻细胞Notch-1的表达，并影响其配体delta-like-1的表达。
英文摘要：
Objective To study the juxtacrine mechanism of Notch-1 in human U251 glioma cells. Methods The expression level of Notch-1 and ligand delta-like-1 in solitary U251 cells and neighboring U251 cells was detected by immunofluorescence histochemistry assay. Notch-1 receptor Of U251 cells was blocked, and the expression level of delta-like-1 mRNA was analyzed using SYBR real-time PCR in different time groups. Results The average fluorescence intensity of neighboring U251 cells was higher than that of solitary cells. The delta-like-1 mRNA level of U251 cells in receptor-blocked groups was significantly lower than that in unblocked group. The level of delta-like- 1 mRNA was descending in a time-dependent manner. Conclusion Notch-1 probably stimulates the expression of notch-1 in neighboring U251 cells via juxtacrine mechanism and influences the expression of Notch-1 ligand delta-like-1.
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