在进行辐射致DNA损伤中,DNA浓度和剂量率是两个重要的因素。利用γ射线在不同的剂量率下对不同浓度的质粒DNA pUC19水溶液进行了辐照,利用凝胶电泳对辐照后的样品进行分析。结果表明,在同一剂量下被辐照的DNA样品,随浓度的降低DNA损伤越来越严重;通过分析软件和理论公式得到的每个DNA上的双链断裂数随着DNA浓度的变化呈现为非线性趋势。
As two factors, the changes of concentration of DNA and dose rate are of significance in the study of DNA damage induced by irradiation. The influence of DNA damage induced by γ ray at different concentration of DNA under different dose rate was investigated. The result of gel electrophoresis indicate that DNA molecules were damaged severely as the concentration of DNA decreasing. The number of double strand breakages per DNA were obtained by the software of Alpha Innotech and theoretical analysis. It shows that the numbers of double strand breakages per DNA presented nonlinear feature as the concentration of DNA decreasing, and the dose rate had little influence on DNA damage.