中文摘要：

锌指蛋白ZEB1（zinc finger E-box binding homeobox 1）是一种在不同物种中相对保守的转录因子,尤其在发生肿瘤的组织中具有较高的表达水平。为了证实禽类锌指蛋白ZEB1在新城疫病毒（Newcastle disease virus,NDV）感染中的作用,本研究扩增获得了鸡源ZEB1的基因序列,并通过生物信息学软件对不同物种的ZEB1基因进行了同源性比对。通过Real-time PCR技术测定NDV感染DF-1细胞、CEF细胞以及SPF鸡后ZEB1的表达量变化,证实NDV感染可使细胞中的ZEB1含量显著升高。但是在细胞中过表达ZEB1对NDV的增殖和蛋白表达没有明显的影响,其生物学意义有待进一步研究。

英文摘要：

Zinc protein ZEB 1 （ zinc finger E-box binding homeobox 1 ） is a transcription repressor, which is highly conserved in different species. To determine the correlation of ZEB1 with Newcastle disease virus（NDV） infection, the gene of chicken ZEB1 was PCR-amplified from DF-I cells and used for phylogenetic and bioinformatic structure analysis. The expression of ZEB1 in NDV- infected DF1 cells, CEF cells and SPF chickens were examined in Real-time PCR. The results showed that NDV infection introduced high expression of chicken ZEB l, suggesting ZEB 1 protein might play a role in the life cycle of NDV. Nevertheless, higher expression of ZEB 1 did not affect the growth and protein expression of NDV. Therefore, the biological role of Zinc protein ZEB 1 need to be further studied.