中文摘要：

目的：分析慢性乙型重型肝炎（以下简称慢重肝）患者外周血各亚群淋巴细胞绝对值的特点。方法：慢重肝患者61例，乙肝引起的肝硬化26例，慢性乙型肝炎21例，健康志愿者10例。采用流式细胞仪检测各自外周血淋巴细胞CD3^＋、CD4^＋、CD8^＋、NK细胞（CD3^-／CD16^＋／CD56^＋）、NKT细胞（CD3^＋／CD16^＋CD56^＋）等亚群表达百分比，计算各淋巴细胞亚群绝对值，并进行统计学分析。结果：与乙肝引起的肝硬化、慢性乙型肝炎患者以及健康志愿者相比，慢重肝患者CD3^＋、CD4^＋及CD8^＋T淋巴细胞的绝对值均明显降低（P〈0．01或P〈0．05）；与存活者相比，慢重肝死亡患者外周血淋巴细胞、CD3^＋、CD4^＋、CD8^＋、NK细胞、NKT细胞绝对值均有一种降低的趋势但尚未达到统计学意义。结论：慢重肝患者存在一定程度的细胞免疫功能紊乱，可能与其预后存在一定的相关性。

英文摘要：

Objective：To investigate the characteristics of peripheral circulating lymphocyte subsets in severe hepatitis patients with chronic HBV infection（SHPCHI）. Methods： The numbers of circulating lymphocyte subsets, including CD3^＋, CD4^＋ and CD8^＋ , NK cells （CD3^- /CD16^＋/CD56^＋ ）, and NKT cells （CD3^＋/CD16^＋ CD56^＋ ）, were determined in SHPCHI （n = 61 ）, patients with chronic hepatitis B （CHB, n = 21 ）, patients with cirrhosis （ LC, n = 26） and healthy volunteers （ n = 10） by flow cytometry. The absolute values of each lymphocyte subset were calculated and statistically analyzed. Results： The absolute numbers of circulating CD3^＋ , CD4^＋ and CD8^＋ T cells were significantly reduced in SHPCHI group compared with those in the other 3 groups （P〈0.01 or P〈0.05）. There was a decreasing trend in the absolute numbers of peripheral CD3^＋ , CD4^＋ , CD8^＋ and NK cells in non-surviving SHPCHI compared with surviving SHPCHI,but the decrease was not statistically significant. Conclusion： SHPCHI has an disorderd cellular immune function, which might be related to the prognosis of SHPCHI.