中文摘要：

内切β-N-乙酰氨基葡萄糖苷酶H（Endo H）是一类识别并切割与糖肽和糖蛋白的天冬氨酸残基连接的寡聚糖上N-乙酰氨基壳糖核心的糖苷酶,可用于糖组学中大规模糖基化位点的鉴定。克隆阿维链霉菌来源的endo H,成功构建p MA0911.1-Endo H重组质粒,并转化到枯草芽孢杆菌WB600中。发酵液经疏水层析和离子交换层析等技术手段得到目的蛋白Endo H。通过重组Endo H高效率酶切RNase B及对鸡卵清标准糖蛋白（OVA）的N-糖链结构的鉴定,表明重组Endo H在糖组学N-糖链结构研究中具有良好的应用前景。

英文摘要：

Endo-β-N-acetylglucosaminidase H（ Endo H） is a glycohydrolase which cleaves the β-1,4- glycosidic bonds of the Nacetylglucosamine core of oligosaccharides and leaves one N-acetylchitobiose attached to the asparagine residue of the glycoprotein. It can be used to identify the glycosylation sites. In this study,we cloned the endo H gene from the Streptomyces avermitilis,then subcloned into expression vector p MA0911. 1. We expressed Endo H in Bacillus subtilis WB600. The fermentation broth was purified by hydrophobic chromatography and ionic exchange,and relatively purified Endo H was obtained. Utility of Endo H in digestion of RNase B and identification of N-glycan structure in OVA indicated the potential of recombinant Endo H in research of N-glycan and glycomic.