中文摘要：

以熔融石英毛细管为免疫分析载体材料,以肿瘤标志物甲胎蛋白（AFP）为模型蛋白,将AFP捕获抗体固定于毛细管,AFP和AFP的辣根过氧化物酶（HRP）标记抗体分别注入毛细管进行孵育,形成双抗体夹心结构。注入化学发光底物液后,HRP催化底物液发光,产生的光信号通过成像仪转化为图片。对图片进行灰度值分析,建立了血清甲胎蛋白的定量免疫分析检测法。当捕获抗体稀释倍数为100,酶标抗体稀释倍数为200,化学发光曝光时间为20 s时,本方法可在3.1-50 ng/m L浓度范围实现甲胎蛋白测定,检出限为2.7 ng/m L。对20例人血清临床实际样本进行检测,结果令人满意,加标回收率在96.0%-106.7%之间。本方法样本消耗少、材料简单、检测成本低、不依赖大型仪器、适用性广,具有较好的临床检验应用前景。

英文摘要：

A fused quartz capillary was taken as the carrier for the immunoassay of the tumor marker alpha fetal protein（AFP） as a model protein.The capture antibody of AFP was immobilized in capillary.Based on a sandwich immunoassay format,the antigen and corresponding horseradish peroxidase（HRP） labeled antibodies were introduced into the capillary for online incubation.After the injection of the chemiluminescence substrate,the chemiluminescence signals were captured by the CCD camera and changed into figures.The results could be read by gray level analysis of the figures.When the capture antibody was diluted 100 times,the enzyme labeled antibody was diluted 200 times,and the exposure time was 20 s,the detection range of AFP was 3.1-50 ng/m L and the critical concentrations of 20 ng/m L clinical applications were covered.The limit of detection was 2.7 ng/m L.Twenty clinical samples were tested with fine accuracy.The recoveries of the spiked samples were between 96.0% and 106.7%.In the assay,the sample consumption was low and the experimental material was simple and cheap.The method does not require large instruments,and is appropriate for clinical application