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水杨酸对厚皮甜瓜采后病害及苯丙烷代谢的影响
  • ISSN号:0578-1752
  • 期刊名称:中国农业科学
  • 时间:2012.3.3
  • 页码:584-589
  • 分类:S435.111.4[农业科学—农业昆虫与害虫防治;农业科学—植物保护]
  • 作者机构:[1]甘肃农业大学食品科学与工程学院,兰州730070
  • 相关基金:国家自然科学基金项目(31071835)、高等学校博士学科点专项科研基金项目(1101.02.006.199)
  • 相关项目:过氧化氢在硅诱导厚皮甜瓜果实抗病反应中的积累与调控模式研究
中文摘要:

【目的】探讨采后水杨酸处理对厚皮甜瓜果实采后病害的抑制效果和苯丙烷代谢的增强作用。【方法】以‘玉金香’厚皮甜瓜为试材,用4mmol·L1-水杨酸呆后常温浸泡10min,测定处理对粉红单端孢(TrichotheciumTOSeum)损伤接种果实病斑直径和自然发病率的抑制,以及对果实苯丙烷代谢关键酶活性和产物积累的影响。【结果】水杨酸处理不仅明显降低了果实损伤接种T.roseum的病斑直径,而且还显著减轻了常温贮藏期间果实的自然发病率。水杨酸处理明显提高了苯丙氨酸解氨酶(PAL)、4-香豆酰-辅酶A连接酶(4CL)和肉桂酸羟化酶(C4H)的活性,处理后6d,其C4H和4CL活性分别比对照高1.0和2.8倍,10d时PAL活性为对照的1.6倍。此外,处理还显著提高了果实的总酚以及木质素含量,处理后4d和10d果实木质素和总酚含量分别高出对照30.6%和30.0%,但处理对类黄酮含量影响不大。【结论】采后水杨酸处理能够通过诱导厚皮甜瓜的苯丙烷代谢增强果实对采后病害的抗性。

英文摘要:

[Objective] The effects of salicylic acid (SA) dipping on postharvest disease resistance and phenylpropanoid pathway in muskmelon fruit was investigated in this paper. [ Method ] Muskmelons (Cucumis melo L. cv.Yujinxiang) were dipped at 4 mmol'L1 of SA for 10 min. The inhibition effect of SA dipping on the lesion diameter of fruits inoculated with Trichothecium roseum and natural incidence of melons were investigated during storage at room temperature. The key enzyme activities and substances accumulation ofphenylpropanoid pathway in fruits were also detected. [Result] SA significantly decreased the lesion diameter of fruits inoculated with T. roseum, and the natural incidence of fruits during storage at room temperature. The treatment increased the activity of phenylalanine ammonia-lyase (PAL), 4-coumarate: CoA ligase (4CL) and cinnamate-4hydroxylase (C4H) in fruits. The activity of C4H and 4CL was 1.0 and 2.8 times higher in treated fruits than that in the control after 6 days of treatment, and PAL activity was 1.6 times of the control after 10 days of treatment. SA significantly enhanced the content of total phenolic and lignin. The content of lignin and total phenolic was 30.6% and 30.0% higher in treated fruits than that in the control after 4 and 10 days of treatment; however, no significant difference was found in flavanoid content between the treatment and the control. [Conclusion] Postharvest SA treatment could increase the resistance in melon fruits against postharvest diseases by inducing phenylpropanoid pathway.

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期刊信息
  • 《中国农业科学》
  • 中国科技核心期刊
  • 主管单位:中华人民共和国农业部
  • 主办单位:中国农业科学院 中国农学会
  • 主编:万建民
  • 地址:北京中关村南大街12号中国农业科学院图书馆楼4101-4103室
  • 邮编:100081
  • 邮箱:zgnykx@caas.cn
  • 电话:010-82109808 82106279
  • 国际标准刊号:ISSN:0578-1752
  • 国内统一刊号:ISSN:11-1328/S
  • 邮发代号:2-138
  • 获奖情况:
  • 中国期刊方阵“双高”期刊,第三届中国出版政府奖提名奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),英国食品科技文摘,中国北大核心期刊(2000版)
  • 被引量:85620