中文摘要：

目的：探讨下丘脑局部兴奋性在孕期摄食限制（PFR）所致子代雌性大鼠下丘脑-垂体-肾上腺（HPA）轴高应激敏感性编程中的作用。方法：建立大鼠PFR模型,孕20d处死部分母鼠取胎鼠。余自然生产,断奶后给予持续高脂饮食饲养,17周时处死一半动物。余于17-20周持续给予不可预测性慢性刺激（UCS）后处死。取雌性动物血清和下丘脑,检测相关指标。结果：PFR组下丘脑超微结构出现损伤,AVP、GAD65、vGLUT2mRNA水平降低,vGLUT2/GAD65比值升高（P〈0.05）。UCS前,PFR组血清ACTH浓度、下丘脑CRH mRNA水平降低,vGLUT2/GAD65比值升高（P〈0.05）。UCS后,PFR组血清ACTH增长率及下丘脑AVP mRNA水平、vGLUT2/GAD65比值均高于对照组（P〈0.05）。结论：PFR诱导的宫内下丘脑局部高兴奋性参与了子代雌性大鼠成年后HPA轴高应激敏感性编程。

英文摘要：

Objective：To investigate the role of hypothalamic irritability induced by the prenatal food restriction（PFR）in the stimulated sensitivity of the hypothalamic-pituitary-adrenal（HPA）axis in female offspring rats.Methods：PFR model was established by reducing 50%food administration of the pregnant rats since pregnant day 11.Then,the rats were killed under anaesthesia at pregnant day 20.The left pregnant rats were kept until spontaneous labor.All the female offspring were fed with high fat diet.Then,half of the rats were killed,while the left were underwent unpredictable chronic stresses（UCS）from postnatal week 17 to 20and killed as well.The bloodand hypothalamus were collected.Serum adrenocorticotropic hormone（ACTH）,hypothalamic mRNA level of hypothalamus corticotrophin-releasing hormone（CRH）,arginine vasopressin（AVP）,vesicular glutamate transporter 2（VGluT2）,glutamic acid decarboxylase 65（GAD65）,and ultrastructure of fetal hypothalamus were detected.Results：Abnormal changes in ultrastructure were observed in fetal hypothalamus.The mRNA levels of AVP,GAD65,vGLUT2 were decreased,while the vGLUT2/GAD65 ratio was increased.Before UCS,lower ACTH serum concentration and CRH mRNA level,as well as increased vGLUT2/GAD65 ratio,were detected in PFR group.Then,the rate of the serum ACTH rise,the AVP mRNA level and vGLUT2/GAD65 ratio were elevated after UCS.Conclusion：PFR stimulated the sensitivity of the HPA axis in female offspring rats through boosting the hypothalamic irritability.