中文摘要：

目的：通过对观察丹酚酸A预处理对局灶性脑缺血再灌注损伤大鼠ICAM-1表达的影响，探讨丹酚酸A预处理对缺血再灌注急性期脑损伤的保护作用机制。方法：丹酚酸A预处理3天后采用线拴法建立大鼠脑缺血再灌注模型，不同时间点（0，1，4，12，24h）取脑组织，冰冻切片，荧光免疫组化法检测缺血区脑微血管内皮ICAM-1的表达。以尼莫地平作为阳性对照药。结果：模型组与空白对照组相比ICAM-1表达明显升高（P〈0．05）。丹酚酸A各组ICAM-1表达均低于相应时间点模型组（P〈0．05）。结论：丹酚酸A预处理可降低局灶性脑缺血再灌注损伤大鼠ICAM-1表达的影响，提示阻抑粒细胞粘附作用是其脑保护作用机制之一。

英文摘要：

Objective ：To study the effect of Salvianolic acid A in ICAM-1 expression in focal cerebra ischemia-reperfusion in rats. Methods ：Establish focal cerebral ischemia reperfusion model in rats with suture method after pre-treated with Salvianolic acid A for 3 days. In different time courses（0,1,4,12,24 hours）. Frozen section of brain tissue was detected by fluorescent-immunohistochemistry technique to test the expression of ICAM-1 on the microvascular endothelium of the ischemic brain region. Normal brain tissue was studied as negative control. Nimodipine was used as positive control. Results： The focal cerebral ischemia reperfusion model was well established according to the expression of ICAM-1 compare with control （ P 〈 0.05 ）. In any time course, the expression of ICAM-1 is significant decreased by Salvianolic acid A compare with model（P 〈0. 05）. Conclusion：Salvianolic acid A can decrease the expression of ICAM-1 of brain tissue after ischemia reperfusion in rats ,which shows a protective effect of brain by depress the adhesion of neutrophil granulocytes on endothelium of ischemic brain region.