中文摘要：

WRKY 抄写因素在植物涉及对关於生命、不能生活的压力的反应的规定。米饭 WRKY82 基因(OsWRKY82 ) 的全身的 cDNA 克隆从从 Magnaporthe grisea 感染的叶子产生的一个 cDNA 图书馆被孤立。OsWRKY82 在一些 1 701 bp 包含了一个全部开的读物框架，并且被预言编码由二个 WRKY 领域组成的 566 氨基酸残余的多肽，有 C2H2 的一个锌手指主题的各个，属于 WRKY 亚群我。OsWRKY82 从蜀黍与那些在氨基酸水平分享了高身份二色， Hordeum vulgare，和 Zea mays。OsWRKY82 的抄本水平在茎，叶子，和花是相对更高的，并且不太充满谷物。它被接种与 M 导致。grisea 和 Rhizoctonia solani。然而，在不兼容的 rice-M 的可诱导的表示。grisea 相互作用在兼容相互作用比那更早、大。OsWRKY82 的表示由甲基 jasmonate 和 ethephon 是起来调整的，而水杨酸酸没在它的表示上施加效果。而且， OsWRKY82 在酵母展出了 transcriptional 激活能力。另外， OsWRKY82 抄本能被伤害并且热震惊，然而并非由 abscisic 酸，寒冷，高咸度和脱水导致。由对比，赤霉素压制了 OsWRKY82 的表示。这些显示那 OsWRKY82 多样地压力可诱导的基因对关於生命、不能生活的压力正在作出回应，并且可以被 jasmonic acid/ethylene-dependent 发信号对不能生活的压力涉及对病原体和忍耐的防卫反应的规定小径。

英文摘要：

WRKY transcription factors are involved in the regulation of response to biotic and abiotic stresses in plants. A full-length cDNA clone of rice WRKY82 gene （OsWRKY82） was isolated from a cDNA library generated from leaves infected by Magnaporthe grisea. OsWRKY82 contained an entire open reading frame in length of 1 701 bp, and was predicted to encode a polypeptide of 566 amino acid residues consisting of two WRKY domains, each with a zinc finger motif of C2H2, belonging to the WRKY subgroup I. OsWRKY82 shared high identity at the amino acid level with those from Sorghum bicolor, Hordeum vulgare, and Zea mays. The transcript level of OsWRKY82 was relatively higher in stems, leaves, and flowers, and less abundant in grains. It was induced by inoculation with M. grisea and Rhizoctonia solani. However, the inducible expression in incompatible rice-M. grisea interactions was earlier and greater than that in compatible interactions. The expression of OsWRKY82 was up-regulated by methyl jasmonate and ethephon, whereas salicylic acid exerted no effects on its expression. Moreover, OsWRKY82 exhibited transcriptional activation ability in yeast. Additionally, OsWRKY82 transcripts could be induced by wounding and heat shocking, but not by abscisic acid, cold, high salinity and dehydration. By contrast, gibberellin suppressed the expression of OsWRKY82. These indicate that OsWRKY82 is a multiply stress-inducible gene responding to both biotic and abiotic stresses, and may be involved in the regulation of defense response to pathogens and tolerance against abiotic stresses by jasmonic acid/ethylene-dependent signaling pathway.