中文摘要：

以MA培养基为基础，向无铁培养基中添加不同质量浓度的FeCl3对对数生长期的铜绿微囊藻进行接种培养，比较不同Fe^3＋浓度下，铜绿微囊藻的生长、活性及产毒状况。结果表明，无Fe^3＋时，藻的生长、细胞活性和产毒受到抑制；Fe^3＋浓度增大时，有利于藻的生长，且当Fe^3＋质量浓度为0．42mg／L时，培养42d后细胞密度达到最大（4．5×10^10个／L）。过高或过低浓度的Fe^3＋均会抑制藻细胞活性和毒素的产生。Fe^3＋质量浓度为0．04mg／L时，脱氢酶活性较好；质量浓度为0．10mg／L时，适宜藻毒素MCLR的生成，且单位生物量藻细胞的MCLR产量可达0．32mg。研究表明，Fe^3＋浓度对藻细胞完整性的影响不大，而且藻细胞的最佳生长条件并不是其最佳产毒条件。

英文摘要：

In order to investigate the effect of iron on the growth of Microcystis aeruginosa, this paper is to make an investigative study over the effect of the iron concentration on the cell activity and its integrity, the toxin production. As is known, water bloom caused by the mass rearing of algae is likely to lead to a serious detriment to the surrounding water environment. For our research purpose, we have adopted MA culture media as growth media at different concentrations of FeCl3 and the algae cells in logarithmic growth phase inoculated into the growth media. During the growth under different iron concentrations, it is necessary to measure the cell density, integrity, activity of dehydrogenase, as well as MCLR content. The testing and measuring resuhs can then be mainly inspected and determined by cell accounting, neutral red staining method, TTC-dehydrogenase assay and HPLC. The results of our investigation indicate that the cell activity and toxin production tend to be noticeably inhibited without iron in the growth media. However, iron concentrations were found with no influence on the cell integrality, for neutral red staining rate was found to be finally below 3 % basically. In so doing, on the 24th day after the inoculation, algae cells would come into logarithmic growth phase. However, iron concentrations were found to have little effect on the cell density before the logarithmic growth, though they may have more and more noticeable influence on the cell densities after that. The increase of Fe^3＋ concentration proves to be advantageous to the growth of algae cells, whose cell density tends to reach their maximum value of 4.5×10^10 cells/L when the Fe^3＋ concentration was 0.42 mg/L. Whereas the dehydrogenase activity of algae cells is likely to get slower under lower or higher Fe^3＋ concentration with the optimum value of 0.04 mg/L, the toxin production can be inhibited under a lower or higher Fe^3＋ concentration with the optimum value being 0.10 mg/L, which can even produce 0.32 mg MCLR per 10^1