采用PKO固体培养基从东祁连山高寒草地中分离出具有解磷能力的菌株33株,对其进行继代培养,得到13株功能稳定的解磷菌,采用2种方法测定其解磷能力。13株解磷菌在PKO平板上8 d后,解磷圈D/d值为1.21~4.01;在液体摇瓶中,其解磷率在0.13~11.842 mg/g。其中,菌株2-2-1具有较高的解磷率,解磷效果在1%水平下显著高于其他菌株,具有较大的应用潜力。
33 phosphorus-solubilizing bacteria were isolated from soil in eastern Qilian Mountains by PKO solid medium.In which,13 phosphorus-solubilizing bacteria had stable phosphorus-solubilizing ability measured with two methods.The ratio of D/d values ranged from 1.21 to 4.01 after 8 days in PKO medium and the ratio of dissolving phosphorus varied from 0.13 mg/g to 11.842 mg/g in liquid cultivate.In which,2-2-1 showed stronger ability of dissolving phosphorus.At 1% level,2-2-1 was significantly different with others.This strain had great potential for applications.