中文摘要：

建立了二级处理出水中肠道病毒的巢式PCR检测方法。在2006年7月至2007年2月期间分别用第一次PCR和巢式PCR方法对西安市北石桥污水净化中心的二级处理出水中的肠道病毒进行了检测，两种检测方法的阳性率分别为12．5％和87．5％。另外，还对二级处理出水中的肠道病毒含量和温度、pH值等理化指标的关系作了分析。

英文摘要：

This study introduced detection method of enteroviruses from secondary effluent by nested-PCR. From July 2006 to February 2007, enteroviruses were detected from the secondary effluent samples of Xi＇an Beishiqiao Wastewater treatment center. Using PCR and nested-PCR, positive sample rate was 12.5% and 87.5% respectively. In addition, the relationship between the concentration of enteroviruses and the temperature, pH of the water and so on were analyzed.