通过对5份花生(中国3份和印度2份)种质资源在水培缺铁条件下各种生理性状的研究,明确花生耐铁的生理或农艺指标,为花生耐缺铁种质资源的筛选和利用提供理论和实践依据。结果表明,缺铁明显影响品种(中花5号、远杂9102和ICG 11855)的生物产量;7d内中花8号、远杂9102和ICG 12672培养液的pH值下降3个单位,而中花5号和ICG11855仅下降2个单位。缺铁处理25d后,中花5号、远杂9102和ICG 11855幼叶的叶绿素含量、SOD、POD和CAT活性下降非常明显,而中花8号和ICG 12672幼叶的叶绿素含量、SOD、POD和CAT活性基本维持不变,这说明缺铁对中花8号和ICG 12672的叶绿素含量降低和细胞膜伤害较小。从膜氧化产物MDA的变化值也印证了缺铁对中花8号和ICG 12672的细胞膜伤害较小。综上所述,认为中花8号和ICG 12672对缺铁不敏感,而中花5号、远杂9102和ICG 11855对铁敏感,同时表明利用水培技术研究花生耐铁是一种简单有效的方法。根据相关分析,选择缺铁处理25 d后的叶绿素含量作为耐缺铁筛选的指标。
In order to investigate physiological responses of peanut varieties to iron deficiency,in the nutrient solution instead of calcareous soils,five accessions of peanuts,including three cultivars from China and two cultivars from India were controlled in 0 or 30mmol/L Fe of nutrient solutions.Some traits were analyzed in 0,12 and 25 days,including plant growth,chlorophyll concentration,capacity of the roots to acidify the medium,and SOD,POD,CAT,MDA.The results showed that iron deficiency significantly affected the whole plant biomass in Zhonghua No.5,Yuanza 9102 and ICG11855.pH value decreased at least 3.5 units during the measure period(7 days),in Zhonghua No.8,Yuanza 9102 and ICG 12672,but slightly exceeding two units in Zhonghua No.5 and ICG 11855.After 25 days of treatment,they significantly decreased in total chlorophyll concentration of leaves and SOD,POD,CAT activities in Zhonghua No.5,ICG 11855 and Yuanza 9102.However,compared to the CK,Zhonghua No.8 and ICG 12672 maintained at the same level.Those results identified that iron deficiency could not be harmful to Zhonghua No.8 and ICG 12672 in chlorophyll and cell membrane,which was testified by MDA concentration(Oxidation outcome of cell membrane).To sum up the above arguments,Zhonghua No.8 and ICG 12672 are not more sensitive to Iron deficiency than Zhonghua No.5,Yuanza 9102 and ICG 11855.At the same time,it is a simple and effective technique to study peanut iron deficiency using nutrient solutions.Based on correlation analysis,total chlorophyll concentration in leaves after 25 days of treatment could be acted as index for tolerance to iron deficiency.