中文摘要：

目的观察细胞外三磷酸腺苷（adenosine Triphosphate,ATP）对体外培养的U87人脑胶质瘤细胞增殖、侵袭和迁移能力的影响。方法采用MTT法检测不同浓度细胞外ATP对U87胶质瘤细胞增殖的影响;以相同条件下不加ATP作为对照组,以加入100μmol/L ATP作为处理组,采用Transwell细胞侵袭实验检测100μmol/L细胞外ATP对U87胶质瘤细胞侵袭能力的影响;用时间显微镜动态观察100μmol/L的ATP作用下U87胶质瘤细胞的迁移情况。结果高浓度（5 000μmol/L）细胞外ATP对U87胶质瘤细胞增殖有显著抑制作用,较低浓度（50、100、500μmol/L）细胞外ATP对U87胶质瘤细胞增殖无明显影响,5 000μmol/L ATP组与其他浓度组比较,差异有统计学意义（P〈0.05）;Transwell侵袭实验中,处理组细胞跨膜细胞数为（163.83±17.81）明显多于对照组（89.83±13.27）（P〈0.01）;迁移实验中,处理组细胞运动速度为（163.83±17.81）μm/h,对照组细胞运动速度为（89.83±13.27）μm/h,2组比较,差异有统计学意义（P〈0.05）。结论 100μmol/L的细胞外ATP对体外培养的U87胶质瘤细胞的侵袭和迁移具有明显促进作用。

英文摘要：

Objectives To determine the effect of extracellular adenosine triphosphate（ATP） on the growth and invasion of U87 glioma cells in vitro.Methods Cell counting kit-8 was used to detect the proliferation in U87 cells after the treatment of ATP at different concentrations of 0,50,100,200,500 and 5 000 μmol/L.Invasion and migration of U87 cells with 100 μmol/L ATP treatment were examined by Transwell and Time microscopy.Results The proliferation of U87 glioma was inhibited obviously by high concentration of ATP（5 000 μmol/L）.However,there was no significant impact on the proliferation in U87 cells by a lower amount of extracellular ATP（50,100 and 500 μmol/L）.The invasion and migration ability of U87 cells were significantly enhanced by the using of 100 μmol/L ATP.Conclusion Extracellular ATP can significantly enhance the ability of invasion and migration in U87 cells.