中文摘要：

目的分析基质金属蛋白酶-9（MMP-9）在小鼠结核性脑膜炎病理生理过程中的表达特点及其意义。方法侧脑室注射结核分枝杆菌H37RV悬液的16只小鼠作为模型组，注射等量0．9％氯化钠溶液的16只小鼠作为对照组，30d后处死。分别进行脑组织结核分枝杆菌培养、病理学观察，明胶酶谱法检测MMP-9活性，检测血脑屏障通透性和脑组织含水量，免疫荧光染色脑组织MMP-9、胶原纤维酸性蛋白（GFAP）和整合素aM（OX-42）。采用t检验比较两组间的差异。结果模型组小鼠注射菌量为（1．271±0．111）×10。菌落形成单位（cfu）／只，感染30d后脑组织匀浆培养出结核分枝杆菌，菌量为（4．900±1．407）×10。cfu／mL，光学显微镜下见蛛网膜下腔和脑室扩张，大量炎性细胞浸润。模型组小鼠脑组织MMP-9条带累积吸光度（A）值为478214-19932，对照组为10082±3544，差异有统计学意义（f=3．728，P=0．010）。模型组小鼠脑组织伊文斯兰（EB）含量为（11．8±3．6）bLg／g，对照组为（4．7±3．4）／tg／g，差异有统计学意义（t=2．887，P=0．028）。模型组小鼠脑组织含水量为0．849土0．035，对照组为0．775±0。037，差异有统计学意义（t=2．925，P=0．026）。免疫荧光染色显示感染后小鼠脑组织高表达MMP-9、GFAP和OX-42，且MMP-9与GFAP和0X-42均明显重合。结论MMP-9在结核性脑膜炎小鼠脑组织中活性增强，并参与破坏血脑屏障，导致组织水肿、炎性细胞渗出，小胶质细胞-星形胶质细胞网络参与MMP-9的分泌。

英文摘要：

Objective To analyze the characteristics and significance of matrix metalloproteinase-9 （MMP-9） expression in the pathophysiological processes of tuberculous meningitis in mice. Methods Sixteen mice were intracerebroventricularly injected with H37RV suspension as the model group. Meanwhile, the other 16 mice were injected with 0. 9% sodium chloride solution as the control group. Thirty days later, all mice were decapitated and the brain tissue were respectively used to for Mycobacterium tuberculosis （M. tuberculosis） incubation, pathological changes observation, MMP-9 activity detection by zymography, blood-brain-barrier permeability and moisture content detection, and immunofluorescence stain of MMP-9, glial fibrillary acidic protein （GFAP） and integrin aM （OX-42）. The t test was used to compare the differences between the two groups. Results Every experimental mouse was injected with （1. 271＋0. 111）X 10^6 colony-forming units （cfu） M. tuberculosis. Thirty days later, the amount of M. tuberculosis in brain tissue homogenates was （4. 900!1. 407） X 104 cfu/mL, and the hematoxylin and eosin staining showed dilatation of subarachnoid and ventricular and infiltration of a large number of inflammatory cells. The cumulative absorbance （A） of MMP-9 bands of brain tissue was 47 821 4-19 932 in the model group and 10 082--4-3 544 in the control group. The difference was statistically significant （t~3. 728, P=0. 010）. The evans blue （EB） content of brain tissue was （11.8＋ 3.6） /xg/g in model group and （4. 7 ± 3. 4） μg/g in control group. The difference was statistically significant （t=2. 887,P=0. 028）. The moisture of brain tissue was 0. 849±0. 035 in model group and O. 775±0. 037 in control group. The difference was statistically significant （t=2. 925, P=0. 026）. The immunofluorescence staining showed that the infected brain tissue expressed high degrees of MMP-9, GFAP and OX-42. And MMP-9 was overlapped with both GFAP and OX-42 obviously. Conclusions The ac