中文摘要：

目的探讨Notch1对骨髓源性脂肪祖细胞（BMAPCs）向脂肪细胞分化的影响。方法无菌取sD大鼠股、胫骨骨髓制作细胞悬液，差速贴壁法培养，流式分析检测细胞表面标志表达；实时荧光定量聚合酶链式反应（real-timeqPCR）检测脂肪相关基因和Notch信号分子表达。Notch1基因沉默实验分为空载体组和Notch1小RNA干扰组。Westernblotting检测干扰效率；STEMPRO Adipogenesis Differentiation Kit行细胞成脂诱导。结果BMAPCs表达CD34、CD44、CD45及CD90，且表达6种脂肪相关基因及Notch信号分子；成脂诱导促进Notch1mRNA表达（P〈0．01）；Notch1小RNA干扰抑制Notch1蛋白表达（P〈0．05）。Notch1小RNA干扰组的Cebpa、Lpl和Ppqγ、Slc2a4与Fabp4 mRNA表达均较对照显著降低，而pread1mRNA表达显著增加（P〈0．05-P〈0．01）；Notch小RNA干扰显著抑制脂肪细胞百分率（P〈0．05）。结论Notch1基因沉默抑制BMAPCs向成熟脂肪细胞分化。

英文摘要：

Objective To explore the effects of Notch1 on the adipocyte differentiation of bone marrow adipocyte progenitor cells （BMAPCs）. Methods The femoral and tibial bone marrow of SD rats was sampled in a sterile manner to prepare cell suspension, which was cultivated by differential adhesion method. The cell surface marker expression was analyzed and detected by flow analysis; the molecular expression of adipose related genes and Notch signaling was detected by means of real-time qPCR. Notch1 gene silencing experiment was divided into non-carrier group and Notch1 small RNA interference group. The interference efficiency was detected by Western blotting; cell fat induction was conducted by STEMPRO Adipogenesis Differentiation Kit. Results BMAPCs expressed CD34, CD44, CD45 and CD90, and six fat-related genes and Notch signaling molecule; fat induction promoted the expression of Notch1 mRNA （P 〈 0.01）; Notch1 small RNA interference inhibited the expression of Notch1 protein （P 〈 0.05）. The levels of Cebpa, Lpl and Ppaγ, Slc2a4 and Fabp4 mRNA in Notch1 small RNA interference group were significantly lower than those in the control group, while the level of preadl mRNA increased greatly（P 〈 0.05 -P 〈 0.01）; Notch small RNA interferencesignificantly inhibits the percentage of fat cells （P 〈0.05）. Conclusion Notch1 gene silencing inhibits the BMAPCs from differentiating into mature fat cells.