中文摘要：

目的采用氢核磁共振（1H-NMR）代谢组学技术对不同生产厂家的阿胶酸水解成分进行差异性比较。方法对5个不同生产厂家的阿胶进行酸水解,然后进行1H-NMR分析。对所得的1H-NMR图谱进行化学成分归属指认,并结合相关软件进行多元统计分析,找出差异性化学成分。结果从阿胶1H-NMR谱中指认出17种化学成分;通过对A、C、D、E厂家生产的阿胶与B厂家生产的阿胶进行比较分析,找出了相应的差异性成分,主要包括异亮氨酸、羟脯氨酸、精氨酸、亮氨酸、苯丙氨酸、缬氨酸、赖氨酸和乙酰丙酸等。结论 1H-NMR代谢组学方法可用于不同厂家阿胶化学成分差异性的分析,为阿胶的质量控制提供新方法和新思路。

英文摘要：

Objective The variability of the acid hydrolysis ingredients of E′jiao from different manufacturers was analyzed with the 1H-NMR-based metabolomics approach. Methods E′jiao from five different manufacturers was hydrolyzed using acid and then analyzed using 1H-NMR. The component belongings were recognized and analyzed combined with relevant software for the multivariate statistical analysis, and then difference components were figured out. Results Seventeen substances were recognized in the 1H-NMR profiling. After comparing E′jiao from manufacturers A, C, D, and E with that from manufacturer B respectively, the difference components including isoleucine, hydroxyproline, arginine acid, leucine, phenylalanine, valine, lysine, and levulinate were confirmed. Conclusion 1H-NMR-based metabolomic study on E′jiao from different manufacturers could be used to analyze the difference components, and provide the new way and reasonable idea for quality control study of E′jiao.