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3种诱变因子对链霉菌Snea253-GL8菌株的诱变效果

ISSN号：1000-3924
期刊名称：《上海农业学报》
时间：0
分类：S432.45[农业科学—植物病理学;农业科学—农业昆虫与害虫防治;农业科学—植物保护] S565.1[农业科学—作物学]
作者机构：[1]沈阳农业大学植物保护学院,北方线虫研究所,辽宁沈阳110866, [2]沈阳农业大学生物科学技术学院,辽宁沈阳110866
相关基金：博士点基金（20112103110006）; 国家自然科学基金（31471748）; 现代农业产业技术体系建设专项（CARS-04-PS13）

作者：朱峰[1], 田成丽[1], 陈井生[1,2], 段玉玺[1], 陈立杰[1]

关键词：大豆根系, 南方根结线虫, 内源激素, 根结, Soybean roots, Meloidogyne incognita, Endogenous hormones, Knots

中文摘要：

通过酶联免疫法（ELISA）测定南方根结线虫侵染大豆根系后,根结部位赤霉素（GA）、玉米素核苷（ZR）、生长素（IAA）、脱落酸（ABA）和茉莉酸甲酯（JA-Me）5种内源激素的含量变化,从而探索寄主对线虫侵染的激素应答反应。研究结果显示：在根结形成过程中,根结内5种内源激素含量均发生变化,其中JA-Me和ZR在线虫侵染发育的28d内持续高于对照;在接种线虫5d内,根结内IAA含量显著高于对照,而ABA则显著低于对照;在接种5d后IAA含量一直低于对照;ABA只在接种14d左右显著高于对照,而GA只在接种21d左右显著高于对照,其他时间ABA和GA与对照基本一致。分析上述5种内源激素间平衡关系表明：IAA/ABA和JA-Me/ABA的比值变化明显,ZR/ABA和GA/ABA的比值平稳。研究结果暗示IAA、ABA和JA-Me在线虫侵染大豆形成根结过程中发挥了更重要的作用。

英文摘要：

Root- knot nematodes（ RKN,Meloidogyne spp.） induce the formation of giant cell,namely root- knot,by directing the normal root cell of soybean plant into nematode- feeding cell. To investigate the role of hormone alternation in this process,contents of IAA,ABA,JA- Me,GA and ZR were monitored by enzyme-linked immunoassay（ ELISA） method. Results showed that contents of all these hormones were changed during the formation of giant cell in soybean roots. Among the hormones,the contents of JA- Me and ZR were consistently higher than those of control within 28 days after RKN inoculation. IAA content was significantly higher than that of control whereas ABA content was significantly lower than that of control within 5 days after inoculation. After that,IAA content was significantly lower than that of control,meanwhile contents of ABA and GA were consistent to those of control basically,except at 14 thday and 21 stday after inoculation,in which the contents of ABA and GA were higher than that of control respectively. Analysis of the five endogenous hormonal ratio changes showed that IAA / ABA and JA- Me / ABA ratio changed significantly,ZR / ABA and GA / ABA ratio were stable. These data indicated that IAA,ABA and JA- Me played important roles on formation of root- knot during nematode infection.Our results also assisted in finding the physiological response mechanism of soybean responding to nematode infection,and to provide a new strategy for preventing and treating of root knot nematodes.

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