中文摘要：

目的为了评价BCG初免-单次重组腺病毒Ad5-CEAB加强的免疫策略诱导小鼠产生的免疫效应。方法在前期工作的基础上,制备并纯化获得了高滴度的重组腺病毒Ad5-CEAB,然后以小鼠为动物模型,采用BCG初免-Ad5-CEAB加强的异源初免-加强的免疫策略对动物进行免疫,通过测定小鼠淋巴细胞抗原刺激液中IL-12和IFN-γ的含量,测定小鼠血清IgG抗体含量以及小鼠肺盥洗液中sIgA的浓度来评价BCG初免-单次重组腺病毒Ad5-CEAB加强免疫策略的免疫效应。结果 BCG初免-Ad5-CEAB加强免疫后,经抗原刺激后的小鼠淋巴细胞会分泌高水平的IL-12和IFN-γ,这两种细胞因子的浓度均显著高于对照组。BCG初免-Ad5-CEAB加强后,还可以有效的刺激小鼠分泌IgG抗体,血清IgG抗体浓度显著高于对照组。另外,小鼠肺盥洗液中sIgA的浓度也显著高于对照组。结论采用BCG初免-单次Ad5-CEAB加强的异源初免-加强的免疫策略能够有效的刺激小鼠产生较强的免疫应答反应。

英文摘要：

Tuberculosis（TB）remains an enormous health burden worldwide.To date,Mycobacterium bovis Bacillus Calmette Guerin（BCG）is the unique anti-TB vaccine available for humans,which provides an important but limited protection from the Mycobacterium tuberculosis（Mtb）infection.It is therefore an urgent need to develop better vaccines and vaccination strategies to prevent the spread of Mtb infection.Heterologous prime-boost vaccination strategies using both BCG and novel anti-TB vaccines have been demonstrated to induce robust immune responses than BCG alone.We have previously demonstrated that a recombinant adenoviral vector Ad5-CEAB co-expressing CFP10,ESAT6,Ag85 Aand Ag85Bof Mtb was able to induce robust antigen-specific immune responses in mice.In the present study,we examined immunological effects of Ad5-CEAB in the mice primed with BCG and boosted with a single dose of the virus via an intranasal route.Results demonstrated that this vaccination strategy could effectively induce strong antigen-specific mucosal and humoral immune responses.These immune responses were characterized with an increased productions of cytokines（IL-12 and IFN-γ）,increased concentration of secretary IgA（sIgA）in bronchoalveolar lavage fluid（BALF）and serum IgG in mice in comparison with mice in BCG group.These data suggested that the regimen of BCG prime-single dose of Ad5-CEAB boosted strategy was novel for inducing antigen-specific immune responses in response to Mtb antigens in vivo,which warrants for further development of adenoviral-based vaccine against Mtb infection.