中文摘要：

内切葡聚糖酶（endoglucanases）是光肩星天牛幼虫肠道的主要纤维素消化酶。本研究以光肩星天牛内切葡聚糖酶的同工酶AgEG2为靶分子，从随机多肽噬菌体展示库中筛选与AgEG2有亲和活性的短肽，通过3轮筛选，短肽序列TPHRSPL出现频率为33．7％，而且展示该短肽的噬菌体均对AgEG2有很高的结合能力。进一步合成短肽TPHRSPL，并对肠道纤维素酶提取液进行了Western分析，结果表明该短肽能特异结合内切葡聚糖酶的同工酶AgEG1和AgEG2，而与粗酶液中其它蛋白组分均无结合特性。表明筛选获得的短肽TPHRSPL对光肩星天牛内切葡聚糖酶具有特异结合亲和性。该短肽为研究光肩星天牛纤维素酶的特性及开发天牛的生物防治制剂奠定了基础。

英文摘要：

Endoglucanases are the main cellulolytic enzymes in the gut of Anoplophora glabripennis. In this study, random peptide phage display technology was employed to screen peptides that bound the AgEG2, a member of endoglucanase isozymes. Phage clones displaying peptide TPHRSPL accounted for 33.7% of the selected phage population after three rounds of screening, and showed higher phage recovery than the other clones in the binding assay. Peptide TPHRSPL was chemically synthesized and tested for its binding activity to AgEG2. The synthetic peptide exhibited high binding specificity for AgEG1 and AgEG2. This indicated that peptide TPHRSPL had the affinity to the endoglucanase of A. glabripenni, which could be used to study the biological role of the enzyme in the gut, and had the potential to be developed into biolog- ical control agents of A. glabripenni.