提取空心莲子草的浸提液,以牧草植物黑麦草、白茅、高羊茅为试验材料,通过测定幼苗体内抗氧化系统中的POD、SOD、APX、MDA含量以及可溶性糖含量,来探讨空心莲子草的化感作用机制。结果表明,浸提液对3种牧草植物根系及叶片POD和SOD活性的影响有明显的一致性,与对照相比,提高了白茅和高羊茅体内POD的活性,而黑麦草体内的POD活性却有所降低,提高了高羊茅和黑麦草体内的SOD活性,而白茅体内的SOD活性却明显下降。浸提液处理显著提高了3种牧草植物根系APX的活性,而叶片中APX的活性均呈下降趋势。高羊茅根系MDA含量较对照有所升高,白茅和黑麦草根系中的MDA含量随着处理浓度的增大而增大,但含量仍低于对照。在叶片中MDA含量在3种牧草植物中的含量均低于对照。可溶性糖含量在根系和叶片的个别处理中有升高的现象,但所有结果表明浸提液处理并未造成3种牧草植物幼苗体内MDA和可溶性糖含量明显的趋势性的增加,表明浸提液并没有对牧草植物的生长产生胁迫作用。
The extraction of Alternanthera philoxeroides was extracted,Lolium perenne Linn.,Imperata cylindrica(L.) Raeusch.,Festuca elata Keng ex E.Alexeev were used as experimental materials,the antioxidant system including the activities of POD,SOD,APX,as well as the content of malondialdehyde(MDA) and soluble sugar were measured to study the mechanism of allelopathic properties of Alternanthera philoxeroides.The results showed that the change of POD and SOD in root were coincidence with that in leaf,the POD in Imperata cylindrica and Festuca eleta Keng ex E.Alexeev were increased when treatment with extraction when compared with the control,while the POD in Lolium perenne Linn.were decreased;the SOD in Festuca elata Keng ex E.Alexeev and Lolium perenne Linn.were increased,while decreased in Imperata cylindrica(L.) Raeusch..The APX in root of three plant were increased,while it was decreased in leaf.The content of MDA in Festuca elata Keng ex E.Alexeev have increased in some degree,the content in Festuca elata Keng ex E.Alexeev and Lolium perenne Linn.were increased with the concentration of extraction,but they were still lower than that in the control.The content of soluble sugar in all the plants were very lower exception less treatment,but all of the results showed that extraction did not cause obvious increasement of MDA and soluble sugar of three forage plant seedlings,indicating that the extraction has no stress on the growth of forage plants.