中文摘要：

以陆地棉低酚棉品种豫无620为材料，在接种黄萎病菌后24h、48h和72h提取叶片蛋白质，采用双向电泳技术研究棉花在黄萎病菌胁迫条件下叶片蛋白质组的变化。结果表明，棉苗在黄萎病菌胁迫下，24h、48h和72h三个时间的叶片蛋白质图谱与未接种对照组均存在显著差异，病菌胁迫下的棉苗叶片被诱导产生大量新的蛋白质，而且在三个时间均出现了DB1、DB2和DB3差异蛋白点。通过MALDI—TOF—MS分析和数据库检索，发现DB1、DB2和DB3分别与DEAD／H box RNA helicase、丝氨酸蛋白酶抑制剂和ODR-3蛋白具有70％、42％和85％的同源性，推测这些蛋白可能在棉花对黄萎病的抗性反应中发挥作用。

英文摘要：

The interaction between cotton and Verticillium dahliae is a field of importance in molecular biology of plant disease resistance. However, there is no report on proteome of cotton under Verticillium dahliae stress. Two-dimensional electrophoresis was employed to analyze the proteome from leaves of cotton glandless variety Yuwu 620 at 24 h, 48 h and 72 h, after inoculating Verticillium dahliae. The results showed that there were significant differences between inoculated plants and control in the proteomic map. After infection of the pathogen, a lot of new proteins were induced to express in the cotton seedlings. At the time of 24 h past inoculation, 88 new proteins were detected, and at 48 h and 72 h, 97 and 101 proteins appeared, respectively. Besides, some of proteins showed intensity differences between inoculated plants and control. Three spots, DB1, DB2 and DB3, were all detected at three stages post inoculation. Analyzing via MALDI-TOFMS and Mascot software, the three differential expression proteins were partly homologous to DEAD/H box RNA helicase （at 70%）, serine proteinase inhibitor （40%） and ODR-3 protein （85%）, respectively. The protein spot DB1 might have NBS domain and have important function in signal transduction pathway and the immune regulation of host. DB2 belonged to potato proteinase inhibitor I family. It would accumulate in plant as plant was infected by microbes, and could inhibit the proteolytic enzymes of pathogens. DB3 belonged to a subunit of G protein. This protein might combine with receptor to activate some signal transduction pathway. It is likely that they play an important role in the resistance responses to Verticillium dahliae.