中文摘要：

目的：研究miR-152分子对NK细胞杀伤JEG-3细胞效应的影响。方法：在滋养细胞肿瘤细胞系JEG-3细胞中分别转染pre-miR-152（实验组）,Cy3标记的pre-miRNA-control（阴性对照）,空白对照（NC）为未转染的JEG-3细胞。转染48h进行NK细胞的细胞毒测定,观察各组NK细胞对JEG-3细胞的杀伤效应。结果：与对照组相比,实验组NK细胞对JEG-3细胞的平均杀伤率显著增高（P〈0.05）,且随着效靶比的增高,杀伤率也增大。空白对照组与阴性对照组之间的NK细胞杀伤能力无显著差异（P〉0.05）。结论：miR-152可以提高NK细胞对滋养细胞的杀伤作用。

英文摘要：

Objective：To investigate the effect of miR-152 on NK cell mediated cytolysis in JEG-3 cells.Methods： The JEG-3 cells were transfected with pre-miR-152（test group）,Cy3 dye labeled pre-miRNA-control（negative control）,respectively.JEG-3 cells transfected with nothing were blank control（NC）.48 hours after transfction,cells were used for NK cell cytotoxicity assay to investigate the NK cell mediated cytolysis in JEG-3 cells.Results： After pre-miR-152 transfection,the lyticactivity of NK cells toward JEG-3 cells was dramatically enhanced compared with that in the control groups at each E/T ratio（NK cells to JEG-3 cell ratio,P0.05）,and the cytotoxicity increased proportionately to an increase in the E/T ratio.There was no difference between negative control group and blank control group（P0.05）.Conclusion： This study reveals that overexpression of miR-152 could lead to increased NK cell mediated cytolysis in JEG-3 cells,suggesting that miR-152 may function as an immune system enhancer through up-regulating NK cell mediated cytolysis of host cells.