中文摘要：

背景：目前,大量研究者致力于寻求可制备出具有优良孔隙、力学性能佳、生物相容性好的三维多孔支架材料方法,其中3D打印技术的快速发展为此提供了较好的技术支持。目的：采用3D打印技术制备丝素蛋白/胶原蛋白复合支架,并对其进行表征。方法：采用3D打印技术制备丝素蛋白/胶原蛋白复合支架,设置丝素蛋白与胶原蛋白的质量比分别为4∶2、4∶4,检测两种复合支架的孔隙率、吸水膨胀率、力学性能及孔径。将第3代大鼠骨髓间充质干细胞接种于两种复合支架上,接种13 d时进行MTT检测,接种14 d时进行苏木精-伊红染色和扫描电镜观察。结果与结论：①4∶2复合支架组的孔隙率、孔径、吸水膨胀率均大于4∶4复合支架组（P〈0.05）,两组复合支架的弹性模量比较差异无显著性意义;②随着接种时间的延长,两组复合支架上的骨髓间充质干细胞数量逐渐增加,4∶2复合支架组细胞数量始终多于4∶4复合支架组（P〈0.05）;③4∶2复合支架内部细胞较多且分布均匀,4∶4复合支架内部细胞较少且分布不均匀;④结果表明,将丝素蛋白与胶原蛋白以质量比4∶2混合,采用3D打印技术制备的丝素蛋白/胶原蛋白复合支架具有良好的理化性能和细胞相容性。

英文摘要：

BACKGROUND： Searching for a porous three-dimensional（3D） scaffold holding good porosity, mechanical property and biocompatibility has become a hot spot, in which, 3D printing technology also plays apart. OBJECTIVE： To prepare silk fibroin/collagen scaffolds using 3D printing technology and detect its performance. METHODS： Silk fibroin/collagen scaffolds were constructed using 3D printing technology, and the silk fibroin/collagen mass ratio was 4：2（group A） and 4：4（group B）, respectively. The porosity, water absorption expansion rate, mechanical properties and pore size of the composite scaffolds were detected. The passage 3 rat bone marrow mesenchymal stem cells were seeded onto the two scaffolds. The cell proliferation was detected using MTT assay at 13 days of culture, and the cell morphology was observed by hematoxylin-eosin staining and scanning electron microscope at 14 days of culture.RESULTS AND CONCLUSION： The porosity, pore size, and water absorption expansion rate of group A were significantly larger than those of group B（P〈0.05）, while the elasticity modulus showed no significant difference between groups. Bone marrow mesenchymal stem cells on the two scaffolds increased gradually with time, especially in the group A（P〈0.05）. Abundant cells distributed evenly in the group A, while few cells distributed unevenly in the group B. These results suggest that the 3D printed scaffolds composed by silk fibroin/collagen mass ratio of 4：2 holds good physicochemical performance and cytocompatibility.